To understand the population composition of endophytic fungi (EF) from hyperaccumulators seeds as well as their possible ecological role in host plants' heavy metal (HM) tolerance and accumulation,the culturable EF from the seeds of two HM hyperaccumulators Arabis alpina and Chenopodium ambrosioides were isolated and further identified based on their morphological characteristics and molecular analysis.The HMs tolerance capacity was assessed through the growth rate measuring on PDA plates which contained different contents of HMs.The results showed that both the diversity and the colonization rate (CR) of EF in the seeds of two hyperaccumulators were lower,and the most dominant EF in them was different,too.The CR of A.alpine and C.ambrosioides were 74.0％ and 42.0％,and the Shannon Index of them were 0.78 and 1.35,respectively.They were lower than those from the plant growing in non-extreme environment.HMs tolerance tests indicated that the isolates of dominant genera/species showed better HMs tolerance capacity than that of the other genera/species isolates (with higher ratio of resistant isolates),and some of them showed Cd toxicity excitatory.The result of minimum inhibitory concentration (MIC) tests indicated that the strain FXZ2 could tolerate lead,zinc and cadmium as high as 40 mmol/L,60 mmol/L and 220 mmol/L,respectively.These strains may play an important role in improving host plants stress tolerance and affecting their HM accumulation.
hyperaccumulatorseedendophytic fungidiversityheavy metal tolerance
NETL
NSTL
万方数据
维普
