This study was conducted using gas production technique in vitro which mixed the rumen fluid with artificial saliva and keeping the feed sample under constant temperature and anaerobic environment to simulate rumen fermentation system.In this way,we studied the fermentation characteristics of wheat straw (WS) mixed with spraying corn bran (SCB),soybean hulls (SH),orange peel (OP) and apple pomance (AP) at rations of 0 ∶ 100,25 ∶ 75,50 ∶ 50,75 ∶25 and 100 ∶ 0 in vitro.The results showed that the fermentation parameter HM and B of WS in vitro increased significantly and the parameter lag reduced gradually after combining with four unconventional feeds.The A ∶ P of combination of WS and SCB was lower than WS (P<0.05).The combination of WS and unconventional feeds could improve its fermentation ability effectively due to the mixed complementary effects of nutrition.The best combination ratio of WS with other feeds were WS ∶ SCB(75 ∶ 25) 、WS ∶ OP(50 ∶ 50) 、WS ∶ AP(75 ∶ 25) 、WS ∶ SH(75 ∶ 25) in terms of the gas production associated effects value.The ratio of propionic acid of VFA increased significantly with the increase of proportion of SCB in the combination of WS and SCB,because the propionic acid had the higher energy conversion efficiency than acetic acid,so it should be more suitable for fattening livestock to promote its feed efficiency.