Changes of oxidative stress and antioxidant enzyme activity of Mesembryanthe-mum crystallinum Linnaeus in response to different concentrations of seawater
A pot experiment was conducted by trea-ting Mesembryanthemum crystallinum L. with six concen-trations of seawater ( 0, 20. 00％, 40. 00％, 60. 00％, 80. 00％ and 100. 00％, respectively ) to analyze the growth pattern, oxidative stress and antioxidant enzyme ac-tivity every 30 d. Compared with no seawater treatment, growth was not inhibited in the treatments with seawater concentrations less than 60％, the contents of H2 O2 and O2.- were less than 10. 00μmol/g and 0. 15μmol/g, and the contents of malondialdehyde ( MDA) and relative electric conductivities were less than 27. 00 μmol/mg and 53. 70％, respectively. At the same time, the activity of superoxide dismutase ( SOD) increased over growth period, and peroxidase ( POD) activity increased twice. The activity of catalase ( CAT) was less than 1. 00 U/g. The most obvious positive growth stimulation was observed in 20. 00％ seawater treatment. When seawater con-centration was more than 60. 00％ , positively related growth inhibition was observsed, which were shown as sharply in-creased contents of H2O2 and O2.- in M. crystallinum L., increased MDA and relative conductivities, and decreased activi-ties of SOD and POD. CAT activity kept stable in 80. 00％ seawater treatment, but increased sharply in 100. 00％ treatment. The results indicated that M. crystallinum L. could grow under whole seawater irrigation and 20. 00％ concentration was the optimum. When seawater concentration was more than 60. 00％, stress resistance decreased gradually. Sea water stress in-duced oxidative stress.
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维普
