Analysis of WRKY gene family based on full-length transcriptome and min-ing of salt stress candidate genes in Vicia faba
The WRKY gene is a plant-specific transcription factor gene that regulates plant growth and development and stress response.In order to identify the WRKY gene family members of Vicia faba,reveal their ev-olutionary relationship and mine candidate WRKY genes re-lated to salt stress,in this study,the WRKY transcription factor genes were identified and analyzed by bioinformatics methods based on the full-length transcriptome(nine sam-ples)and second-generation transcriptome sequencing(27 samples)of Vicia faba,and the candidate VfWRKY genes related to salt stress were excavated by homologous gene alignment of Arabidopsis thaliana.The results showed that 53.84 Gb data were obtained from the full-length transcriptome sequencing of Vicia faba,and 58 885 transcript sequence informa-tion was finally obtained through alignment and correction.A total of 113 WRKY family members were identified based on the full-length transcriptome of Vicia faba,with amino acid numbers of 153-737 aa and isoelectric points of 4.84-9.87,all 113 WRKY family proteins were localized in the nucleus.According to the phylogenetic characteristics of Arabidopsis WRKY family,the VfWRKY gene family was divided into three groups:group 1(38 VfWRKY),group 2(61 VfWRKY),and group 3(14 VfWRKY).Motif 1 and Motif 3 were characteristic motifs of the VfWRKY gene family and corresponded to the WRKY domain,which were relatively conserved during evolution.The VfWRKY gene family was mainly enriched in three pathways:plant-MAPK signaling pathway,plant-pathogen interaction pathway and spliceosome pathway.Fourteen candidate WRKY genes related to salt stress were found in Vicia faba by homology alignment,and they were mainly highly expressed in the roots.The results of this study can provide rich reference data for the genetics research of Vicia faba,and also provide gene information for creating new varieties of salt-tolerant Vicia faba.
万方数据
维普
