Effects of exogenous hydrogen peroxide on pea seed germination and seed-ling antioxidant enzyme activity under NaCl stress
Pea seeds were used as test materials to study the effects of exogenous H2O2(0 mmol/L,5 mmol/L,10 mmol/L,20 mmol/L,40 mmol/L,and 80 mmol/L)on the germination,seedling growth and physiological characteristics under 170 mmol/L NaCl treatment.The results showed that compared with CK,seed germination and seedling growth were significantly inhibited under 170 mmol/L NaCl treatment,and both germination index and physiological index were signifi-cantly decreased.Under 170 mmol/L NaCl stress,seed soaking with low concentration of exogenous H2O2 could improve the germination index of pea seeds,promote the growth of seedling roots and buds,promote the accumulation of proline and soluble sugar,decrease the content of malondialdehyde,and increase the activities of SOD,CAT and POD in roots and buds.The results showed that exogenous H2O2 could improve salt tolerance of pea by regulating the accumulation of osmotic adjustment substances and antioxidant enzyme activity.Among them,5 mmol/L H2O2 significantly alleviated the toxicity of NaCl stress on seed germination and seedling growth,and the effect was the best.
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