Cloning and function analysis of JcHDZ28 gene from Jatropha curcas L.
The genes of HD-Zip family are closely related to plant growth and resistance to environmental stress,and are considered to be key factors in crop improvement.In the study,we cloned a HD-Zip family gene from Jatropha curcas L.using RT-PCR technology,and named JcHDZ28.The JcHDZ28 gene contained an open reading frame of 882 bp,and encoded a protein with 293 amino acids.Amino acid sequence analysis showed that JcHDZ28 contained a highly conserved homologus domain and leucine zipper(LZ)motif.Expression profile analysis showed that the relative expression of JcHDZ28 gene was the highest in seeds,and salt stress inhibited the expression of this gene.Subcellular localization analysis showed that JcHDZ28 gene encoded a nuclear localization protein.Overexpression of JcHDZ28 gene increased the sensitivity of transgenic Arabidopsis to salt stress.Under salt stress,the proline content of transgenic plants was significantly lower than that of wild type,and the relative conductivity was significantly higher than that of wild type.The expression of abiotic stress-related genes in JcHDZ28 transgenic plants was also significantly lower than that in wild type.The results of this study can provide a theoretical basis for further elucidating the function of HD-Zip transcription factor gene JcHDZ28 in the growth,development and response to abiotic stress in Jatropha cur-cas L.
万方数据
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