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盐碱地旱生芦苇根际解磷菌株筛选及促生特性

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为进一步挖掘解磷菌菌种资源,研制适合于盐碱地作物的促生菌剂,利用选择性培养基从新疆克孜勒苏柯尔克孜自治州盐碱地旱生芦苇(Phragmites australis)根际土壤中筛选解磷菌株.结合限制性片段长度多态性技术与 16S rDNA测序进行菌株鉴定,并挑选解磷能力突出的优良菌株开展植物接种试验.本试验共筛选到 47 株解磷菌,其中 32 株可溶解无机磷,溶磷量为55.8~722.3 mg/L;37 株可溶解有机磷,溶磷量为11.8~34.4 mg/L.47株解磷菌隶属于 7 个属,以肠杆菌属(Enterobacter)占绝对优势.解磷菌株有效促进了拟南芥植株的生长和根系发育,以芽孢杆菌Bacillus sp.PN-2和类芽孢杆菌Paenibacillus sp.PM-1的促生作用最显著.解磷菌株对盐碱土中小麦地上部分(株高、茎粗、地上部分鲜质量和地上部分干质量)和地下部分(根鲜质量、根干质量)也表现出积极影响,以Enterobacter sp.PM-9和Enterobacter sp.PM-2促生作用最突出.本研究筛选的解磷菌株具有一定的应用潜力,对开发适用于盐碱地的微生物肥料有重要意义.
Screening and growth-promoting characteristics of phosphorus-solubilizing strains from the rhizosphere of Phragmites australis in saline-alkali soil
In order to further explore the resources of phosphorus-solubilizing bacteria and develop growth-promoting agents suitable for crops in saline-alkali land,phosphorus-solubilizing bacteria were screened from rhizosphere soil of dryland Phragmites australis in Xinjiang Kizilsu Kirgiz Autonomous saline-alkali soil.Strain identification was carried out by combining restriction fragment length polymorphism and 16S rDNA sequencing,and the strains with outstanding phosphor-us-solubilizing ability were selected for plant inoculation test.As a result,a total of 47 strains of phosphate-solubilizing bac-teria were screened.Among them,32 strains could dissolve inorganic phosphorus with a phosphorus solubility of 55.8-722.3 mg/L.And37 strains could dissolve organic phosphorus with a phosphorus solubility of 11.8-34.4 mg/L.The 47 phosphate-solubilizing bacteria belonged to seven genera,and Enterobacter was dominant.The phosphate-solubilizing strains could effectively promote the growth and root development of Arabidopsis thaliana,especially Bacillus sp.PN-2 and Paeniba-cillus sp.PM-1.The above-ground part(plant height,stem diameter,fresh weight and dry weight of above-ground part)and underground part(fresh weight and dry weight of root)of wheat in salin-alkali soil were also positively af-fected by the phosphate-solubilizing strain,Enterobacter sp.PM-9 and Enterobacter sp.PM-2 were the most promi-nent.The phosphate-solubilizing strains screened in this study have certain application potential and are of great significance for the development of microbial fertilizers suitable for saline-alkali soil.

phosphorus-solubilizing strainssaline-alkali soilgrowth-promoting effectsmicrobial fertilizer

王继莲、王冬玲、周茜、张甜、李明源

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喀什大学生命与地理科学学院,新疆 喀什 844006

新疆帕米尔高原生物资源与生态自治区重点实验室,新疆 喀什 844006

解磷菌 盐碱土壤 促生作用 微生物肥料

新疆维吾尔自治区自然科学基金项目

2021D01A18

2024

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2024.40(1)
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