Screening of functional genes associated with uterus and ovary develop-ment in Jiangquhai pig based on RNA-Seq technology
The development of uterus and ovary tissues can directly affect the reproductive performance in sows,which in turn has a significant impact on the economic efficiency of the pig breeding industry.To investigate the molecular mechanism of uterus and ovary development in Jiangquhai pigs,we selected three 1-month-old and three 8-month-old pigs for RNA-seq analysis of the uterus and ovary tissues.We performed statistical screening and enrichment analysis using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)to identify differentially expressed genes.Furthermore,we compared these genes with the pig quantitative trait locus(QTL)database to identify poten-tial candidate genes.Our results showed that there were 1 688 differentially expressed genes between the uterine tissue of 1-month-old and 8-month-old Jiangquhai pigs,with 844 genes up-regulated and 844 genes down-regula-ted.There were 3 833 differentially expressed genes in o-varian tissue,of which 2 831 genes were up-regulated and 1 002 were down-regulated.These differentially expressed genes were significantly enriched in processes such as animal or-gan development,tissue development,and cell differentiation.In this study,11 candidate genes associated with develop-mental traits were identified in the uterine tissue,and 31 candidate genes were identified in the ovarian tissue.Among these,six candidate genes were found to be overlapping in both tissues,namely LHFPL1,MTUS2,LIF,RBP4,EPHB2,and TESC.In conclusion,the results provide a theoretical basis for studying the molecular aspects of uterus and ovary de-velopment in Jiangquhai pigs.Additionally,it offers a new research direction for enhancing the reproductive traits of Jian-gquhai pigs through molecular improvement.
万方数据
维普
