Characterization of CBF family in cabbage and the expression analysis of low-temperature-induced genes BoCBF1,BoCBF2a,and BoCBF3
Transcription factor CBF plays an important role in response to low temperature stress and enhancing cold resistance in plants.In order to analyze the amino acid sequence characteristics of CBF family members in cabbage and ex-plore whether the expression of BoCBF genes was induced by low temperature,the whole genome database of cabbage 923 was used to identify BoCBF gene members and analyze the protein physicochemical properties,phylogenetic relationships,gene structures,and gene expression levels under 2℃ low temperature stress.The results showed that a total of seven BoCBF genes were identified and divided into two sub-groups(Ⅰ and Ⅱ)after phylogenetic analysis.The amino acid length of BoCBF proteins ranged from 203 aa to 283 aa,all of which were hydrophilic proteins.BoCBF1,BoCBF2a,and BoCBF3 genes were not expressed in leav-es,flowers,buds and siliques of cabbage 02-12,and the expression levels were low in callus,roots and stems of cabbage 02-12.Transcriptome sequencing and real-time fluorescence quantitative PCR analysis revealed that the expression of BoCBF2b and BoCBF2c genes was not induced by low temperature in cold-intolerant cabbage 923 and cold-intolerant cabbage D9,while BoCBF2a gene was most rapidly induced by low temperature,followed by BoCBF1 and BoCBF3 genes.The relative expression levels of BoCBF1,BoCBF2a,and BoCBF3 genes reached the maximum at 3-6 h after low tempera-ture stress and decreased sharply after reaching the maximum,and reached the lowest at 24 h.The results of the present study provide a basis for the subsequent study on the mechanism of BoCBF1,BoCBF2a and BoCBF3 genes in response to low temperature stress in cabbage.
cabbageCBF gene familylow temperature stressgene expression
万方数据
维普
