Identification of candidate genes for watermelon leaf yellowing based on BSA and transcriptome analysis
The leaf is one of the important functional organs of plants.It is not only the main place for photosynthesis of plants,but also can be used as an important morphological marker in breeding.As a morphological marker,leaf color can be used not only for removing hybrids at the seedling stage,but also for determining seed purity.In this study,F1,F2,and BC1 populations were created by hybridization,and the mutant homozygous inbred line ly104 in the whole growth period of watermelon was used as the female parent(P1),and the green leaf inbred line w3 was used as the male parent(P2).Genetic analysis showed that leaf yellowing was controlled by a single recessive gene.The bulked segregant analysis(BSA)was used for primary mapping,and genome-wide single nucleotide polymorphism(SNP)markers were developed by restriction-site associated DNA-sequencing(RAD)to construct a high-density genetic map of watermelon.The watermelon leaf yellowing gene was localized on chromosome 2 at 13 950 306-15 517 591 bp(about 1.57 Mb).Watermelon 97103v2 was used as the reference genome and the in-terval contained 24 annotated genes.The transcriptome levels of P1(P1Y),P2(P2G)and yellow leaf(F2Y)and green leaf(F2G)lines in F2 population were analyzed.The results showed that the expression levels of Cla97C02G035950,Cla97C02G036010,Cla97C02G036020 and Cla97C02G036060 in the target interval were significantly different between etio-lated leaves and normal green leaves.These genes might be candidate genes for etiolation of watermelon leaves.The re-sults of this study can lay an important foundation for further analysis of the function and biological characteristics of watermelon leaf yellowing genes.
万方数据
维普
