The establishment of regeneration system of Fragaria viridis Weston
The establishment of an efficient and stable regeneration system of Fragaria viridis Weston is the basis of its genetic transformation system.In this study,the stolon tips of Fragaria viridis Weston were used as explants and placed in differ-ent media for primary culture to obtain test-tube seedlings.The leaf discs of test-tube plantlets with leaf age of 30 days were dedi-fferentiated in the medium containing different ratios of plant growth regulators.The calluses induced by leaf discs were used as materials to explore the effects of different plant growth regulators on adventitious buds.The results showed that the most suitable medium for primary culture of Fragaria viridis Weston was MS+0.2 mg/L N-(phenylmethyl)-9H-purin-6-amine(6-BA)+0.2 mg/L gibberellin A3(GA3).In this medium,the survival rate of Fragaria viridis Weston shoot tips in vitro was the highest and the growth condition was the best.The suitable media for inducing leaf discs dedifferentiation,callus redifferentiation and adven-titious bud rooting were MS+1.5 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D)+2.0 mg/L thidiazuron(TDZ),1/2 MS+1.5 mg/L 6-BA+0.1 mg/L 3-indolebutyric acid(IBA)and 1/2 MS+0.2 mg/L IBA+0.1 mg/L 3-indoleacetic acid(IAA),respective-ly.The callus incidence,redifferentiation rate and rooting rate were 100%,91.0%and 100%,respectively.In this study,the tissue rapid propagation system using the stolon tips of Fragaria viridis Weston as experimental materials and a regeneration system using leaf discs as explants through dedifferentiation and callus redifferentiation were established,which laid a technical foundation for the establishment of its genetic transformation system.
Fragaria viridis Westoncallusregenerationgenetic transformation system
万方数据
维普
