Fine mapping and transcriptome analysis of a narrow leaf mutant gene vrnl9 in mungbean
Leaf is the most important place for mungbean photosynthesis,and its morphological structure affects photo-synthesis,population structure and yield in mungbean.Screening and identification of leaf shape mutants can lay the founda-tion for studying molecular regulation mechanism of leaf development and genetic improvement of leaf shape in mungbean.A narrow leaf mutant vrnl9 was identified from an ethyl methyl sulfonate(EMS)induced Wankelü 3(WK3)mutant library.Phenotype identification,gene mapping and transcriptome analysis for vrnl9 were completed.Phenotype analysis showed that the leaf width,leaf area and petiole length of vrnl9 were significantly decreased compared with wild-type WK3.The main stem of mutant degenerated and the growth period was extended by ten days.In order to map the mutation site,the F2 population constructed by the cross between Sulü16-10 and the mutant vrnl9 was used for genetic analysis and fine mapping.The narrow leaf trait of vrnl9 was controlled by a single recessive nuclear gene(χ2=1.40).The BSA sequencing analysis showed that the mutation site was located within an interval of 0-3.1 Mb on chromosome 9.The narrow-leaf gene vrnl9 was finally narrowed down to a 354.6 kb region between the markers NL-15 and NL-28 by using map-based cloning strategy.Transcriptome analysis revealed that the expression levels of 182 genes in mutant vrnl9 were significantly changed com-pared with wild-type WK3,of which 86 genes were up-regulated and 96 genes were down-regulated.KEGG analysis results showed that the differentially expressed genes were significantly enriched in plant hormone signal transduction,terpenoid backbone biosynthesis,zeatin biosynthesis and other metabolic pathways.These results provide a theoretical basis for cloning vrnl9 and understanding the molecular regulation mechanism of leaf development in mung bean.
万方数据
维普
