Analyzing the autotoxicity of phenolic acids from Lycium barbarum L.leaves
Autotoxicity is one of the main reasons for the formation of continuous cropping barriers,which seriously affects plant growth and food production.The results of previous studies showed that the decrease of soil microbial diversity and the aggravation of soil-borne diseases were the important reasons for the formation of continuous cropping obstacles.But the self-toxic effects and the material basis of residues of Lycium barbarum were still unclear.In order to study the main sources of autotoxins,the effects of leaf,stem and root extracts of Lycium barbarum L.on the growth of Lycium barbarum L.seedlings were studied.The results of concentration gradient test showed that the inhibition effect of leaf extract was higher than that of root,old root bark and stem extract.The growth and photosynthesis of Lycium barbarum L.seedlings were significantly inhibited by adding Lycium barbarum L.leaf powder to the farmland soil without Lycium bar-barum L.planting history.It was proved that the leaves of Lycium barbarum L.had self-toxicity.Combined with UPLC-QT-OF-MS and GC-TOF-MS for metabolome analysis of Lycium barbarum L.leaves at the end of growing season,a total of 112 organic acids,41 alcohols,37 amino acids,28 aldehydes and ketones and 48 sugars were identified.Analysis of 24 kinds of organic acids and derivatives showed that 20 kinds of organic compounds could inhibit the primary root growth of Lycium barbarum L.seedlings.Salicylic acid,phthalic acid,p-hydroxybenzoic acid,and coumarin still showed significant inhibito-ry effects at concentrations as low as 10 μmol/L.UPLC results further showed that long-term continuous cropping led to the accumulation of coumarin,salicylic acid,benzoic acid,ferulic acid and coumaric acid.In addition,the content of total phenolic acids in soil increased significantly with the increase of planting years.These results indicated that phenolic acids could accumulate and induce autotoxicity in the leaves of Lycium barbarum L.under long-term continuous cropping,which provided a basis for further research on the mechanism of continuous cropping failure.
万方数据
维普
