首页|连云港地区野生灵芝生物学特性及基因组特征分析

连云港地区野生灵芝生物学特性及基因组特征分析

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野生灵芝资源在连云港境内有着广泛的分布,本研究使用全基因组重测序技术对具有不同形态特征和生境特点的灵芝基因组进行测序及分析,以期解析连云港地区野生灵芝的生物学特性及基因组特征.结果表明,从不同区域采集的野生灵芝种质资源菌丝具有锁状联合,具备形成子实体的条件,生物学特性存在较大差异;通过主成分分析将 12 株野生灵芝菌株种质资源聚为 3 类,其中第Ⅲ类具有更丰富的遗传多样性;比较单核苷酸多态性(SNP)、插入或者缺失(InDel)在 12 株灵芝菌株基因组中的分布情况发现,第 1 条染色体中的SNP最多,占比为 46.07%,SNP中转换颠换比(Ts/Tv)为 2.03,说明变异以转换为主,插入或缺失导致的基因差异在第 1 条染色体上最多,占总InDel数的 44.31%.研究结果说明,连云港地区灵芝具有丰富的遗传多样性,可以用于进一步挖掘更多的功能基因和进行品种选育.
Analysis of biological characteristics and genomic characteristics of wild Ganoderma lucidum in Lianyungang
Wild Ganoderma lucidum resources are extensively found in Lianyungang.In this study,we utilized whole-genome resequencing technology to examine and analyze the genomes of Ganoderma lucidum with different morpholog-ical and habitat characteristics.The purpose of this study was to unveil the biological and genomic characteristics of wild Ganoderma lucidum in Lianyungang.The results showed that the mycelium of wild Ganoderma lucidum germplasm resources collected from different regions had clamp connection,and had the conditions to form fruiting bodies,and the biological characteristics were quite different.Through principal component analysis,12 wild Ganoderma lucidum germplasm resources were grouped into three categories,and the third category had more abundant genetic diversity.Analyzing the distribution of single nucleotide polymorphism(SNP)and insertion or deletion(InDel)in the genome of 12 strains of Ganoderma lucidum,it was found that the number of SNP on chromosome 1 was the largest,accounting for 46.07%,and the conversion and inversion ratio(Ts/Tv)in the SNP was 2.03,indicating that the variation was mainly conver-sion.The genetic difference caused by insertion or deletion was the highest on chromosome 1,accounting for 44.31% of the total InDel number.These results showed that Ganoderma lucidum in Lianyungang had rich genetic diversity,which could be used to further explore more functional genes and breed varieties.

Ganoderma lucidumbiological characteristicswhole genome resequencingprincipal component anal-ysis

纪伟、苏文英、刘晓梅、任立凯、胡曙鋆、孙潇潇、陈克龙

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连云港市农业科学院,江苏 连云港 222000

青海师范大学,青海 西宁 810000

连云港市园艺蔬菜指导站,江苏 连云港 222000

灵芝 生物学特性 全基因组重测序 主成分分析

江苏现代农业(蔬菜)产业技术体系连云港市财政专项连云港市食用菌全产业链建设项目

JATS2022177QNJJ2209连农复202314号

2024

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2024.40(2)
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