The Bacillus tequilensis KC 121 which isolated from the lead-zinc tailings of Lanping,showed strong inhi-bition activity against Fusarium spp.In this article,its fungal inhibition activity,plant growth promotion effect,and the corresponding mechanism were studied.The sterile filtrate of KC 121 was used to co-culture with pathogenic fungi and co-incubation with pathogenic fungal spores to determine its effects on hyphal growth,conidia production and germination.The maize seeds were soaked with fermenta-tion broth to determine the effects of strain KC 121 on maize seed germination.Finally,the bio-control effect of strain KC 121 against Fusarium and the plant-growth pro-motion effect on maize seedlings were studied by pot exper-iment.The results showed that after co-culture with KC 121,the hyphae of Fusarium were wrinkled and sunken,and conidia production and germination were also inhibi-ted.The fermentation broth of strain KC 121 could promote the growth of taproot by 61％ and bud growth by 162％ during maize seed germination.In the pot experiment,the inhibition effects of KC 121 fermentation broth on root rot caused by Fu-sarium solani and Fusarium sporotrichioides were 66.67％ and 76.00％,respectively,and the dry and fresh weight of maize were increased by 130％ and 90％,respectively.The KC 121 strain promoted the growth of maize seedlings by producing amylase,producing 6.19 ng/ml indole acetic acid(IAA)and increasing the total chlorophyll content.The B.tequilensis KC 121 showed important potential in controlling the Fusarium disease of maize and promoting maize growth.
维普
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