首页|特基拉芽孢杆菌KC 121拮抗玉米镰刀菌的防病促生作用

特基拉芽孢杆菌KC 121拮抗玉米镰刀菌的防病促生作用

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为初步探究 1 株分离自兰坪铅锌尾矿的特基拉芽孢杆菌KC 121 的防病促生效果及作用机理,将其无菌滤液涂布平板与病原菌共培养以及与发酵滤液病原菌孢子共孵育来测定菌株KC 121 对病原菌菌丝生长、产孢数量及孢子萌发的影响;以发酵液浸种试验来测定菌株KC 121 对玉米种子萌发的影响;最后利用盆栽灌根的方法研究菌株KC 121 对玉米镰刀菌根腐病的防治效果及对玉米幼苗的促生作用并探究其机理.结果表明,特基拉芽孢杆菌KC 121 无菌滤液可使镰刀菌菌丝发生皱缩和凹陷,同时抑制了镰刀菌孢子的产生和萌发;浸种试验结果表明,稀释1×103 倍的菌株KC 121 发酵液可以促进玉米种子萌发时主根增长61%,芽长增加162%;在盆栽试验中,菌株KC 121 发酵液原液对腐皮镰刀菌(Fusarium solani)和拟枝孢镰刀菌(Fusarium sporotrichioides)引起的玉米根腐病的防治效果分别为 66.67%和 76.00%,且分别使玉米植株地上部分干质量和鲜质量提高了 130%和 90%.促生机理探究发现特基拉芽孢杆菌KC 121 通过产淀粉酶,并产生 6.19 ng/ml的吲哚乙酸(IAA)和提高玉米幼苗的总叶绿素含量来促进玉米幼苗生长.研究结果显示特基拉芽孢杆菌KC 121 对防治玉米镰刀菌病害及促进玉米生长具有应用潜力.
Disease prevention against corn Fusarium and growth promotion of Bacil-lus tequilensis KC 121
The Bacillus tequilensis KC 121 which isolated from the lead-zinc tailings of Lanping,showed strong inhi-bition activity against Fusarium spp.In this article,its fungal inhibition activity,plant growth promotion effect,and the corresponding mechanism were studied.The sterile filtrate of KC 121 was used to co-culture with pathogenic fungi and co-incubation with pathogenic fungal spores to determine its effects on hyphal growth,conidia production and germination.The maize seeds were soaked with fermenta-tion broth to determine the effects of strain KC 121 on maize seed germination.Finally,the bio-control effect of strain KC 121 against Fusarium and the plant-growth pro-motion effect on maize seedlings were studied by pot exper-iment.The results showed that after co-culture with KC 121,the hyphae of Fusarium were wrinkled and sunken,and conidia production and germination were also inhibi-ted.The fermentation broth of strain KC 121 could promote the growth of taproot by 61％ and bud growth by 162％ during maize seed germination.In the pot experiment,the inhibition effects of KC 121 fermentation broth on root rot caused by Fu-sarium solani and Fusarium sporotrichioides were 66.67％ and 76.00％,respectively,and the dry and fresh weight of maize were increased by 130％ and 90％,respectively.The KC 121 strain promoted the growth of maize seedlings by producing amylase,producing 6.19 ng/ml indole acetic acid(IAA)and increasing the total chlorophyll content.The B.tequilensis KC 121 showed important potential in controlling the Fusarium disease of maize and promoting maize growth.

Bacillus tequilensisFusariumantibacterial mechanismpromoting growth mechanism

吴雅杰、樊炳君、朱国兴、焦钰、张兴丽、王瑞雪、周萍、曹艳茹

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昆明学院农学与生命科学学院,云南 昆明 650214

云南丽江市植保植检站,云南 丽江 674199

特基拉芽孢杆菌 镰刀菌 抑菌机理 促生机理

国家自然科学基金项目云南省地方本科高校(部分)基础研究联合专项省部共建云南生物资源保护与利用国家重点实验室开放课题云南省"万人计划"青年拔尖人才资助项目云南省教育厅科学研究基金项目云南省教育厅科学研究基金项目

316600022018FH001-0032019KF005YNWR-QNBJ-2018-0112021Y7332022Y715

2024

10.3969/j.issn.1000-4440.2024.02.005

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子：1.093
ISSN：1000-4440
年,卷(期)：2024.40(2)
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