Relationship between soil microbial community structure and soil physico-chemical factors of shady slope and sunny slope of tea garden
Illumina MiSeq high-throughput sequencing technology was used to explore the bacterial and fungal community structure characteristics of the shady slope and sunny slope of tea garden,and analysis of the effects of soil physical and chemical factors on the bacte-rial and fungal community structures was conducted.The results showed that the number of bacterial and fungal operational taxonomic units(OTUs)in the shady slope soil was higher than that in the sunny slope soil.At the phylum classification level,the bacterial phyla in the shady slope soil and the sunny slope soil with the highest relative abundance were Acidobacteria and Proteobacteria re-spectively,and the most abundant fungal phylum in both slopes was Ascomycota,with a relative abundance exceeding 55.00%.At the order classification level,the most abundant bacteria and fungi in the shady slope soil and sunny slope soil were different.Species diversity analysis showed that there was no significant difference in species diversity or richness of bacterial and fungal communities in the soils between the shady and sunny slopes of the tea garden.Dif-ferent bacterial species were enriched in the shady slope and sunny slope soils,while the fungal species enriched were similar.Redundancy analysis found that bacteria received greater influence by soil factors than fungi,and soil availa-ble phosphorus content was the most important environmental factor affecting soil bacterial and fungal communities.
tea gardenshady slope and sunny slopesoil microorganismmicrobial community structuresoil physicochemical factor