Effects of dietary supplementation with selenohomolanthionine on the structure and composition of rumen archaea in Shaanbei white cashmere wether goats
In order to clarify the effects of different doses of selenohomolanthionine(SeHLan)on the structure and composition of rumen archaea in Shaanbei white cashmere wether goats,thirty-two Shaanbei white cashmere wether goats with consistent development were selected and divided into four groups randomly.They were fed diets with selenium contents of 0.016 mg/kg(CK),0.300 mg/kg,0.600 mg/kg and 1.200 mg/kg,respectively.After the 70-day feeding experiment,the rumen fluid of all the experimental wether goats was collected,DNA was extracted,and PCR amplification was performed using archaeal-specific prim-ers.The archaeal V4-V5 region was subjected to high-throughput sequencing analysis,and the structure and composition of archaea in the rumen fluid of wethers were analyzed.The results showed that with the increase of SeHLan addition,the α diversity index of rumen fluid archaea showed a trend of decreasing first and then increasing,but the difference of α diversi-ty index of rumen fluid archaea between treatments was not significant.The relative abundance of Euryarchaeota archaea at the phylum level,Methanobacteriaceae archaea at the family level and Methanobrevibacter archaea at the genus level in ru-men fluid of each treatment was close to 80%,and with the increase of dietary SeHLan addition,the relative abundance showed a trend of decreasing first and then increasing.The relative abundance of Euryarchaeota archaea at the phylum lev-el,Methanobacteriaceae archaea at the family level and Methanobrevibacter archaea at the genus level in rumen fluid of wether goats treated with 0.600 mg/kg selenium content diet was significantly lower than that of the other three treatments.In summary,the addition of SeHLan to the diet significantly affected the composition and structure of rumen fluid archaea in Shanbei white cashmere goats.The abundance of rumen methanogens in wethers treated with 0.600 mg/kg selenium content diet was significantly lower than that in other treatments.
selenohomolanthionineShaanbei white cashmere wether goatshigh-throughput sequencing technolo-gyrumenarchaea
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维普
