Genetic diversity analysis of four cultured Xenocypris davidi populations based on mitochondrial Cyt b gene sequence
Xenocypris davidi is one of the main fish species that are proliferated and released into the natural waters of Zhejiang province.To understand the impact of artificial breeding on the genetic diversity of X.davidi and provide basic data for formulation and implementation of prolifera-ting and releasing strategies for X.davidi,the mitochondri-al DNA cytochrome b(Cyt b)gene was used to study the genetic diversity of four cultured populations.The results showed that 157 variation sites were detected in the 1 140 bp sequence that encoded the Cyt b gene,and 21 haplotypes were defined.Among them,the haplotype numbers in Changx-ing,Shuangpu,Balidian and Liling populations were 10,11,7 and 2,respectively.The haplotype diversity ranged from 0.226 to 0.794,and the nucleotide diversity ranged from 0.006 14 to 0.023 86.The Liling population had low genetic di-versity,while the other three cultured populations were characterized by genetic diversity with large haplotype number and nucleotide diversity.The genetic distances among four cultured populations of X.davidi ranged from 0.018 74 to 0.092 74,and the genetic differentiation index was 0.808 63(P<0.01),among which the genetic differentiation degrees between Changxing and Balidian populations were low,and the genetic differentiation degrees between Shuangpu and Liling popula-tions were high.Moreover,genetic differentiation mainly occurred between populations.The study results can provide refer-ence for resource protection and artificial proliferation and releasing of X.davidi at molecular level.
Xenocypris davidicultured populationCyt b genegenetic diversity
万方数据
维普
