首页|南瓜叶黄素基因紧密连锁的InDel分子标记开发及应用

南瓜叶黄素基因紧密连锁的InDel分子标记开发及应用

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本研究旨在开发与中国南瓜(Cucurbita moschata Duch.)叶黄素含量的数量性状基因座(Quantitative trait locus,QTL)紧密连锁且实用性强的分子标记,以加速南瓜的育种进程.前期在F2代群体中定位到与叶黄素含量紧密连锁的主效QTL位点qlut11-a,在其两侧翼分子标记R1 38695 和R2 55819 之间开发了 8 对InDel分子标记.通过对F2代群体及部分高代(F8代)重组自交系株系进行基因型和叶黄素表型分析,明确了InDel分子标记G005310 和G005670 可有效筛选高叶黄素含量和低叶黄素含量材料,并在近等基因系构建中证实了其能用于创制高叶黄素含量的南瓜种质,BC5 F1果实中的最高叶黄素含量约是低叶黄素含量亲本果实含量的 2.8 倍,且占高叶黄素含量亲本果实的 96%.本研究结果为加速南瓜高叶黄素含量种质育种进程提供了更实用的分子标记.
Development and application of closely linked InDel molecular markers of lutein gene in Cucurbita moschata Duch.
To accelerate the breeding progress of Cucurbita moschata Duch.(C.moschata),the study aimed to de-velop molecular markers which are tightly linked with quantitative trait locus(QTL)of lutein content in C.moschata and are highly practicable.Eight pairs of insertion-deletion(InDel)molecular markers were developed between the two flanking molecular markers R1 38695 and R2 58819,based on the previous localization of the primary QTL locus qlut11-a which was tightly linked to lutein content in F2 population.Genotype analysis and phenotypic analysis of lutein content for the F2 population and recombinant in-breed lines(RILs)in part of the high generation(F8)re-vealed that,InDel molecular markers G005310 and G005670 could be used to effectively screen materials with high and low lutein contents.Furthermore,the two InDel molecular markers were proved to be able to culture pump-kin germplasms with high lutein content in the construction of near isogenic line(NIL).The highest lutein content in BC5.F1 fruit was about 2.8 times as high as the lutein content in parent fruit with low lutein content,and the lutein content in BC5 F1 fruit accounted for about 96% of the lutein content in parent fruit with high lutein content.The research results can provide more practical molecular markers for accelerating breeding process of pumpkin germplasms with high lutein content.

pumpkinluteinQTL localizationHPLC determinationInDel molecular marker

徐颖超、张思程、薛舒丹、刘佳黎、朱吉童、孟琦涛、林惠境、聂呈荣、钟玉娟

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广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东 广州 510640

佛山科学技术学院园艺学院,广东 佛山 528231

南瓜 叶黄素 QTL定位 HPLC测定 InDel分子标记

广东省农业科学院食品营养与健康研究中心建设运行经费项目广西重点研发计划项目广东省农业科学院科技人才引进专项广东省蔬菜新技术重点实验室开放基金项目国家自然科学基金项目广东省现代农业产业技术体系创新团队建设项目

XTXM202205桂科AB23026076R2022YJ-YB3012202201321726042022KJ117

2024

10.3969/j.issn.1000-4440.2024.02.017

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子：1.093
ISSN：1000-4440
年,卷(期)：2024.40(2)
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