Effects of exogenous 2,4-epibrassinolide on root growth,physiological charac-teristics and soil enzyme activities of potato under alkaline salt stress
In order to explore the effects of exogenous 2,4-epibrassinolide(EBR)on potato root morphology,physiological characteristics and soil enzyme activities under alkaline salt stress,a pot experiment was carried out with potato"Atlantic"as the test material,and no NaHCO3(CK)and adding 300 mmol/L NaHCO3(T0)were set up.After addition,exogenous EBR was sprayed with 0.25 μmol/L(T1),0.50 μmol/L(T2),1.00μmol/L(T3),2.00 μmol/L(T4),a total of six treatments.The results showed that 300 mmol/L NaHCO3(T0)stress in-hibited the growth,development and physiological characteristics of potato roots,and soil enzyme activities.Exogenous EBR at different concentrations could alleviate the stress of NaHCO3 on potatoes.Compared with T0 treatment,root diame-ter,root surface area,root volume,root tip number,root vigor and root dry weight of T1 treatment-T4 treatment were im-proved.Root relative electrical conductivity,malondialdehyde(MDA)content,hydrogen peroxide content and superoxide anion content decreased during the whole growth period.Proline(Pro)content,superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),urease,sucrase,alkaline phosphatase and dehydrogenase activities were enhanced.Based on the principal component analysis of 19 indexes,the ability of exogenous EBR to alleviate potato alkaline salt stress from high to low was T3>T2>T4>T1>T0.Therefore,1.00 μmol/L exogenous EBR could better improve the growth of potato roots,enhance the stability of biofilm,and improve the activities of antioxidant enzymes and soil enzymes under salt-alkali stress,so as to alleviate the salt-alkali stress.
2,4-epibrassinolidealkaline salt stresspotatoroot morphologyphysiological characteristicssoil enzyme activity