首页|外源2,4-表油菜素内酯对碱性盐胁迫下马铃薯根系生长、生理特性及土壤酶活性的影响

外源2,4-表油菜素内酯对碱性盐胁迫下马铃薯根系生长、生理特性及土壤酶活性的影响

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为探究外源 2,4-表油菜素内酯(EBR)对碱性盐胁迫下马铃薯根系形态、生理特性及土壤酶活性的影响,本研究以马铃薯大西洋为试验材料进行盆栽试验,设置不添加NaHCO3(CK)和添加 300 mmol/L NaHCO3(T0),以及添加 300 mmol/L NaHCO3后喷施0.25 μmol/L(T1)、0.50 μmol/L(T2)、1.00 μmol/L(T3)、2.00 μmol/L(T4)外源EBR,共 6 个处理.结果表明,300 mmol/L NaHCO3(T0)胁迫抑制马铃薯根系的生长发育、生理特性及土壤酶活性.喷施不同浓度外源EBR均能缓解NaHCO3对马铃薯的胁迫,相比于T0 处理,T1 处理~T4 处理的根粗、根表面积、根体积、根尖数、根系活力以及根系干质量均得到提高;整个生育期根系相对电导率、丙二醛(MDA)含量、过氧化氢含量及超氧阴离子含量明显下降;脯氨酸(Pro)含量以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、脲酶、蔗糖酶、碱性磷酸酶、脱氢酶活性增强.对 19 个指标进行主成分分析,依据主成分得分排序,外源EBR对马铃薯碱性盐胁迫缓解能力由高到低为T3>T2>T4>T1>T0.因此 1.00 μmol/L 的外源EBR可更好地改善盐碱胁迫下马铃薯根系的生长,增强生物膜的稳定性,提高抗氧化酶及土壤酶的活性,从而起到缓解盐碱胁迫的作用.
Effects of exogenous 2,4-epibrassinolide on root growth,physiological charac-teristics and soil enzyme activities of potato under alkaline salt stress
In order to explore the effects of exogenous 2,4-epibrassinolide(EBR)on potato root morphology,physiological characteristics and soil enzyme activities under alkaline salt stress,a pot experiment was carried out with potato"Atlantic"as the test material,and no NaHCO3(CK)and adding 300 mmol/L NaHCO3(T0)were set up.After addition,exogenous EBR was sprayed with 0.25 μmol/L(T1),0.50 μmol/L(T2),1.00μmol/L(T3),2.00 μmol/L(T4),a total of six treatments.The results showed that 300 mmol/L NaHCO3(T0)stress in-hibited the growth,development and physiological characteristics of potato roots,and soil enzyme activities.Exogenous EBR at different concentrations could alleviate the stress of NaHCO3 on potatoes.Compared with T0 treatment,root diame-ter,root surface area,root volume,root tip number,root vigor and root dry weight of T1 treatment-T4 treatment were im-proved.Root relative electrical conductivity,malondialdehyde(MDA)content,hydrogen peroxide content and superoxide anion content decreased during the whole growth period.Proline(Pro)content,superoxide dismutase(SOD),catalase(CAT),peroxidase(POD),urease,sucrase,alkaline phosphatase and dehydrogenase activities were enhanced.Based on the principal component analysis of 19 indexes,the ability of exogenous EBR to alleviate potato alkaline salt stress from high to low was T3>T2>T4>T1>T0.Therefore,1.00 μmol/L exogenous EBR could better improve the growth of potato roots,enhance the stability of biofilm,and improve the activities of antioxidant enzymes and soil enzymes under salt-alkali stress,so as to alleviate the salt-alkali stress.

2,4-epibrassinolidealkaline salt stresspotatoroot morphologyphysiological characteristicssoil enzyme activity

李明、颉嘉丽、石铭福、康益晨、张卫娜、刘玉汇、杨昕宇、秦舒浩

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甘肃农业大学园艺学院,甘肃 兰州 730070

甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070

甘肃省农业科学院马铃薯研究所,甘肃 兰州 730070

2,4-表油菜素内酯 碱性盐胁迫 马铃薯 根系形态 生理特性 土壤酶活性

国家自然科学基金项目国家自然科学基金项目国家自然科学基金项目国家现代农业产业技术体系项目甘肃省自然科学基金项目甘肃省自然科学基金项目

322604553206044132201810CARS-09-P1422JR5RA85823JRRA1339

2024

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子:1.093
ISSN:1000-4440
年,卷(期):2024.40(3)
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