茉莉花PAL基因家族的多基因组鉴定与表达分析
Multi-genomic identification and expressional analysis of PAL gene family in Jasminum sambac
张宇航 1谷梦雅 1洪雅萍 1林宏政 1金珊 1叶乃兴1
作者信息
- 1. 福建农林大学园艺学院/茶学福建省高校重点实验室,福建 福州 350002
- 折叠
摘要
为深入探究茉莉花PAL基因家族的功能及在香气成分形成中的作用,本研究以福州单瓣茉莉花、双瓣茉莉花和重瓣茉莉花基因组数据为基础,利用生物信息学方法及实时荧光定量PCR技术,对JsPAL基因家族进行多基因组分析.结果表明,在单瓣茉莉花、双瓣茉莉花和重瓣茉莉花全基因组中分别鉴定出 4 个、1 个和 4 个PAL家族基因,JsPAL家族成员均具有丙氨酸-丝氨酸-甘氨酸(Ala-Ser-Gly)组成的三肽活性中心.共线性分析发现单瓣茉莉花、重瓣茉莉花和拟南芥的PAL基因间的共线性关系与双瓣茉莉花相比更强;系统发育树分析结果显示,Js-PAL基因家族分布在 2 个亚家族中.JsPAL基因启动子区域存在大量与胁迫响应、激素响应、光响应和植物生长相关的顺式调控元件.荧光定量分析结果表明,JsPAL在茉莉花组织中存在特异性表达,多数JsPAL在花中的相对表达量高于其他组织;大多数JsPAL基因在福州单瓣茉莉花中的相对表达量高于双瓣茉莉花;福州单瓣茉莉花中SP_JsPAL2、SP_JsPAL4、MP_JsPAL1 和MP_JsPAL3 在其花朵预开放期(F3)的相对表达量显著高于其他时期,双瓣茉莉花中DP_JsPAL1 在F3 阶段的相对表达量也较高;SP_JsPAL1 和SP_JsPAL2 与 2-苯乙醇和苯乙醛含量呈显著正相关,其中SP_JsPAL1 还与苯甲醛含量呈显著正相关.说明,JsPAL可能在茉莉花的发育中起重要作用,SP_JsPAL2、SP_JsPAL4、MP_JsPAL1、MP_JsPAL3 和DP_JsPAL1 可能对福州单瓣茉莉花、双瓣茉莉花的香气成分形成具有重要的调控作用,SP_JsPAL1 可能对福州单瓣茉莉鲜灵香气的形成具有重要作用.
Abstract
To deeply investigate the function of the PAL gene family in Jasminum sambac and its role in the forma-tion of aroma components,multi-genomic analysis of JsPAL gene family was conducted by bioinformatics methods and quan-titative real-time PCR technology based on the genomic data of single-petal J.sambac,double petal J.sambac and multi-petal J.sambac from Fuzhou.The results showed that four,one and four PAL family genes were identified from the whole genome of single-petal J.sambac,double petal J.sambac and multi-petal J.sambac,respectively.All members of the JsPAL family had a tripeptide active center composed of alanine-serine-glycine(Ala-Ser-Gly).Collinearity anal-ysis revealed that the collinear relation of PAL gene be-tween single-petal J.sambac,multi-petal J.sambac and Arabidopsis thaliana was stronger compared with that of double-petal J.sambac.Analysis of phylogenetic tree showed that the JsPAL gene family was distributed in two subfamilies.There were many cis-regulatory elements that were related to stress response,hormone response,light response and plant growth in the promoter region of JsPAL genes.The results of fluorescence quantification analysis showed that Js-PAL was specifically expressed in J.sambac tissues,and the relative expression of the majority JsPALs in flowers were high-er than in other tissues.The relative expression of the majority JsPAL genes in Fuzhou single-petal J.sambac was higher than in double-petal J.sambac.The relative expressions of SP_JsPAL2,SP_JsPAL4,MP_JsPAL1 and MP_JsPAL3 in the pre-opening stage(F3)of Fuzhou single-petal J.sambac were higher than in other stages,the relative expression of DP_Js-PAL1 in the F3 stage of Fuzhou double-petal Jasminum sambac was also high.SP_JsPAL1 and SP_JsPAL2 were positively correlated with 2-phenylethanol and phenylacetaldehyde contents,and SP_JsPAL1 was also positively correlated with ben-zaldehyde content.The results indicated that JsPALs may play an important role in flower development of J.sambac,and SP_JsPAL2,SP_JsPAL4,MP_JsPAL1,MP_JsPAL3 and DP_JsPAL1 may have important regulatory roles in the formation of aroma components in Fuzhou single-petal J.sambac and double-petal J.sambac.Moreover,SP_JsPAL1 may play an impor-tant role in the formation of fresh spirit aroma of Fuzhou single-petal J.sambac.
关键词
茉莉花/苯丙氨酸解氨酶(PAL)/表达分析/多基因组Key words
Jasminum sambac/phenylalanine ammonia-lyase(PAL)/expressional analysis/multi-genomic引用本文复制引用
基金项目
国家"十四五"重点研发计划项目(2022YFD2101102)
福州茉莉花茶科技与全球重要农业文化遗产联合研究中心专项(KH1501920)
福建张天福茶叶发展基金会科技创新基金项目(FJZTF01)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据