Establishment of haemagglutination detection method for quantification of foot and mouth disease virus based on bispecific nanobodies
In this study,the gene fragments of the nanobody Nb205 against foot-and-mouth disease virus(FMDV)and the nanobody NbRBC48 against chicken aldehyde red blood cell(cRBC)were connected by genetic engineering method to construct the bispecific nanobody Nb205-48.Its relative molecular weight was 3.5×104,and it could react with FMDV and cRBC simultaneously.A new hemagglutination method for the detection of FMDV antigen was established successfully by using Nb205-48.The detection sensitivity of this method was 1 μg/ml,and it had no cross-reaction with other viruses.In addition,the intra-batch and inter-batch repeatability was good.Three batches of FMDV antigen were detected by hemagglutination method and sandwich ELISA method,and the results were similar.In this study,a novel hemagglutination method based on bispecific nanobody Nb205-48 was established.The method had good sensitivity,specificity and repeatability,and was simple,fast and low cost.The detection results were well correlated with the results of traditional FMDV quantitative detection methods.The method constructed in this study provides a new method for detecting the content of FMDV antigen in the production of foot-and-mouth disease vaccine.
foot and mouth disease virusbispe-cific nanobodieshaemagglutination detection method
NETL
NSTL
万方数据
维普
