Effects of formulated feed and frozen fresh fish on the composition and content of amino acids and fatty acids and serum biochemical indices in the muscle of Micropterus salmoides
Extensively feeding with the frozen fresh fish can contaminate the aquaculture water with nutrients and patho-gens and induce disease outbreaks,ultimately reducing production efficiency of Micropterus salmoides.In order to mitigate the problems caused by extensively feeding with fro-zen fresh fish and boost the development of high-quality for-mulated feed,it is vital to evaluate the nutritional value and health status of Micropterus salmoides fed with formulated feed and frozen fresh fish.Therefore,in this study,360 healthy Micropterus salmoides were divided into three experimental groups and fed with formulated feed,frozen fresh fish and mixed feed(the weight of formulated feed and frozen fresh fish accounted for 50%of the total weight of feed,respectively).The mineral element content,the composition and content of amino acids,the composition and content of fatty acids and serum bio-chemical indices of Micropterus salmoides in the three groups were evaluated after feeding for 90 days.The results showed that the contents of sodium(Na),calcium(Ca)and zinc(Zn)in the muscle of Micropterus salmoides fed with formulated feed were sig-nificantly higher than those in the frozen fresh fish group(P<0.05).Compared with formulated feed and mixed feed groups,the percentage of essential amino acids to total amino acids,and percentage of essential amino acid to non-essential amino acid in the muscle of Micropterus salmoides in the frozen fresh fish group were higher.Meanwhile,the amino acid score and chemical score of each essential amino acid,and essential amino acid index in the frozen fresh fish group were higher than those in formulated feed group.The total content of saturated fatty acids in the muscle of largemouth bass in the formulated feed group was significantly higher than that in the mixed feed group and the frozen fresh fish group(P<0.05),but the total content of polyunsaturated fatty acids(PUFA),the contents of eicosapentaenoic acid and docosahexaenoic acid in the frozen fresh fish group were significantly higher than those in the formulated feed group(P<0.05).Compared with the formulated feed group,the n-6 PUFA content of the frozen fresh fish group was lower,the n-3 PUFA content was higher(P<0.05),and the fatty acid quality was better.The serum total protein content and alkaline phosphatase activity in the frozen fresh fish and mixed feed groups were significantly higher than those in the formulated feed group(P<0.05).The lowest glucose content and aspartate aminotransferase activity were observed in the frozen fresh fish group(P<0.05).Therefore,under the conditions of this study,formulated feed affected the nutritional value of muscle and liver health of Micropterus salmoides,it was recommended to refer to the nutritional composition of fresh-frozen fish and further optimize the formulated feed.
Micropterus salmoidesfrozen fresh fishformulated feedamino acidfatty acidserum biochemical index
NETL
NSTL
万方数据
维普
