Full-length transcriptome sequencing analysis of Chimonobambusa sichua-nensis after discoloration of bamboo culm
In order to explore the molecular mech-anism of bamboo culm changing from green to purple un-der light,we collected the purple part(Z),median part(M)and green part(L)of the same bamboo node,ex-tracted RNA,and used PacBio Sequel three-generation full-length transcriptome sequencing technology combined with bioinformatics methods to analyze the full-length transcrip-tome of bamboo barks with different colors.The results showed that a total of 66 961 non-redundant transcripts were ob-tained after three generations of sequencing and data quality control.The length ranged from 500 bp to 3 000 bp,with an average length of 1 389.22 bp.The total length of the sequence was 93.02 Mbp,the N50 was 1 830 bp,and the G+C base content was 49.56%.A total of 56 938 transcripts were successfully annotated based on NR,Swiss-Prot,COG,GO,KEGG,and Pfam databases,accounting for 85.03%of all transcripts.A total of 49 115 transcripts were annotated by GO database,among which catalytic activity,organelle and metabolic process were the items with the most transcripts in molecular function,cellular component and biological process of GO database,respectively.The 28 231 transcripts were annotated by KEGG database,of which the transcripts related to carbohydrate metabolism and gene translation were the most.Combined with GO annotation and KEGG annotation,381 transcripts of 66 961 transcripts were related to the perception,transmission and light regulation of light signals,including red and far-red light receptor proteins PHYA,PHYB and signal pathway proteins PIF3,ELF3,blue light receptor proteins CRY,ZTL and signal pathway proteins COP1,SPA1,ELF3,GI,HY5,etc.The pathways related to plant pigment synthesis,such as flavonoid(including an-thocyanin)metabolism,chlorophyll synthesis and carotenoid synthesis,contained 187 transcripts,44 transcripts and 72 transcripts,respectively.A total of 2 079 transcription factors were obtained from the full-length transcripts,including 18 R2R3MYB transcription factors related to anthocyanin synthesis encoded by 35 transcripts.The number of long non-cod-ing RNAs(LncRNAs)simultaneously annotated in the CNCI,CPC,Pfam,and PLEK databases was 6 359,which was higher than that of other bamboo species such as Xiaoshun bamboo.In this study,we obtained high-quality full-length transcriptome data,red and blue light receptors and light signal transduction proteins related to bamboo discoloration,and transcription factors and LncRNAs related to anthocyanin,chlorophyll and carotenoid synthesis,which provided a basis for further analysis of the mechanism of bamboo discoloration.
Chimonobambusa sichuanensiscolor-changing bamboo culmfull-length transcriptome sequencinglight signal
NETL
NSTL
万方数据
维普
