Identification of ZmZIM family genes and their response to nitrogen in maize
In order to clarify the structure,location,encoded protein properties and response characteristics of maize ZmZIM family genes to nitrogen,this study used TBtools,MEGA X and other softwares to analyze the structure,chromo-some location,cis-acting elements and phylogenetic relationships of maize ZmZIM family genes and the physical and chemi-cal properties,conserved domain and motifs of their encoded proteins.The expression patterns and differences of maize Zm-ZIM family genes were analyzed by combining the transcriptome data of different organs at different development stages of maize and the transcriptome data of maize shoots at four-leaf and one-heart stage under sufficient nitrogen and low nitrogen levels.The results showed that a total of 32 ZmZIM genes were identified from the whole genome of maize,which were mainly distributed on chromosomes 1,2,5 and 7,and no ZmZIM genes were found on chromosomes 8 and 10.The 32 ZmZIM genes could be divided into four sub-classes,and the encoded proteins were composed of 134-467 amino acid residues,all of which were hydrophilic proteins and located in the nucleus.The cis-acting elements in the promoter region of 32 ZmZIM genes were mainly divided into six categories:regulatory elements,light signal response elements,hormone signal response elements,stress response elements,growth and development elements and protein binding sites.At different developmental stages,ZmZIM gene was differentially expressed in different organs of maize.Under sufficient nitrogen and low nitrogen treatments,with the increase of treatment time,the 12 ZmZIM genes in the aboveground part of maize plants had no expression or low relative expres-sion,the relative expression of six ZmZIM genes was high and stable,and the relative expression of the remaining 14 Zm-ZIM genes varied greatly.The relative expression levels of ZmZIM5,ZmZIM16,and ZmZIM31 were generally higher than other genes.Under sufficient nitrogen conditions,the relative expression levels of ZmZIM8,ZmZIM15,ZmZIM20,Zm-ZIM24,ZmZIM29 and ZmZIM31 were generally higher than those under low nitrogen conditions.The results of this study lay a foundation for the screening and utilization of nitrogen efficient absorption and utilization genes in maize.
maizeZIM transcription factorsgene family analysisnitrogen response
万方数据
维普
