Alcohol acyltransferase(AAT)can catalyze the combination of acetyl coenzyme A and terpene alcohols to pro-duce various types of acetate compounds.In order to explore the regulatory role of lavender AAT gene in the synthesis of ester compounds,the cDNA sequence of lavender AAT gene was cloned by reverse transcription PCR using the leaves of lavender strain Zahua as the test material.The length of the cDNA sequence was 1344 bp,and the encoded protein was composed of 447 amino acids,which was a non-transmembrane hydrophilic protein and belonged to the PLN02481 superfamily.The AAT in lavender was closely related to the EgAAT in yellow monkey-faced flower,with a similarity of 60.87%.The expression level of AAT gene was lowest in bracts and highest in petals.At different developmental stages of corolla,the expression of this gene reached the peak at the full-bloom stage.Prokaryotic expression vector pET-28a-AAT was constructed.The optimal expression conditions of the recombinant protein in Escherichia coli Transetta(DE3)were screened.The relative molecular weight of the recombinant protein was about 5.026×104.The recombinant protein existed in the form of inclusion bodies and had the bi-ological activity of catalyzing the synthesis of linalyl acetate from linalool.The results of this study provide a basis for fur-ther verifying the function of lavender AAT gene in the syn-thesis of ester compounds.
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