Transcriptome analysis of the response of heat shock protein encoding genes induced by salicylic acid in tea plants
Salicylic acid is an important signal molecule in mechanism of plant resistance induction.Externally spra-ying salicylic acid can regulate multiple defense-related proteins and improve the resistance of crops.Research on the resist-ance mechanism of tea plants induced by exogenous salicylic acid can explore resistance genes and provide molecular basis for resistance breeding of tea plants.In this study,transcriptome sequencing and analysis were conducted on tea leaves col-lected at 0 h 6 h,12 h,24 h and 48 h of spraying exogenous salicylic acid.The results showed that numbers of differentially expressed genes in tea leaves at 6 h,12 h,24 h and 48 h of spraying exogenous salicylic acid were 9 360,3 399,596 and 115 respectively,604 genes were differentially expressed at each time point after exogenous salicylic acid treatment.Results of KEGG functional en-richment showed that 95,73,121,94 and 154 differentially expressed genes were respectively enriched in plant hormone signal transduction,plant-pathogen interaction,ribosome,spliceosome and carbon metabolic pathways six hours after treat-ment.Among the differentially expressed genes,12 genes of heat shock protein transcriptional factors,40 genes of heat shock proteins and 12 transcriptional factor genes of WRKY family were up-regulated.No up-regulated gene of HSP tran-scriptional factors was found after 48 h of treatment,but 28 HSP genes were upregulated.Expression of genes encoding pathogenesis related protein were up-regulated at the detection stage.The induction effect of exogenous salicylic acid was the most obvious at six hours of treatment and caused many responses of heat shock protein.The results of the study provided a reference for the research of disease-resistance mechanism of tea tree induced by exogenous salicylic acid and molecular breeding for disease resistance of tea tree.
exogenous salicylic acidtea planttranscriptomedifferentially expressed geneheat shock protein
万方数据
维普
