Function of the autophagy-related gene ATG20 in Colletotrichum gloeospo-rioides in Hevea brasiliensis
Colletotrichum gloeosporioides is the dominant race that causes anthracnose on rubber trees,resulting in substantial reduction in rubber production.Autophagy is a common physiological process in plant pathogenic fungi,which is regulated by multiple autophagy related genes(ATG)and plays an important role in stress response,growth and pathoge-nicity of pathogenic fungi.In this study,the ATG20 homologous gene in C.gloeosporioides of rubber tree was cloned and named as CgATG20,and the CgATG20 gene knockout mutant △CgATG20 was constructed.The pathogenicity,spore pro-duction,tissue infection and autophagy of the mutant△CgATG20 were analyzed.The results showed that the pathogenicity,conidium production and plant invasion rate of △CgATG20 were significantly reduced compared with wild type.Under the condition of nitrogen deficiency,the number of autophagosomes in△CgATG20 mycelium was significantly less than that of wild type.The above results indicate that ATG20 plays an important role in the growth and development,pathogenicity and autophagy of C.gloeosporioides,which provides a new target for finding control strategies that can effectively block the de-velopment and infection pathways of pathogenic fungi.
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