Effects of alginate oligosaccharides supplementation on immunity of Exo-palaemon carinicauda
In order to investigate the effects of algi-nate oligosaccharides(AOS)on the immunity and resist-ance of Exopalaemon carinicauda to Metschnikowia bicuspi-date,four treatments were set up,the addition levels of AOS were 0 mg/kg(CK),500 mg/kg(T1),1 000 mg/kg(T2)and 2 000 mg/kg(T3),respectively.The shrimps were fed under the same conditions for 60 days.The results showed that the terminal weight,total weight growth rate and specific growth rate of Exopalaemon carinicauda of T2 treatment were significantly higher than those of CK(P<0.01),and the survival rate of Exopalaemon carinicauda of T3 treatment was significantly higher than that of CK(P<0.01).Compared with CK,the SOD activity in hepatopancreas,ACP activity in hepatopancreas and muscles,AKP activity in hepatopancreas and muscles,and PO activity in hepatopancreas of Exopalaemon carinicauda of T2 treatment were signifi-cantly increased(P<0.01).The relative expression level of LGBP in hepatopancreas of Exopalaemon carinicauda of T1 treatment was significantly higher than that of CK(P<0.01).The relative expression levels of SOD and LGBP in hepato-pancreas of Exopalaemon carinicauda of T2 treatment were significantly higher than those of CK(P<0.01).The relative expression levels of LZM,SR and CTSB genes in hepatopancreas of Exopalaemon carinicauda were not significantly differ-ent from those of CK(P>0.05).The results showed that the addition of 1 000 mg/kg alginate oligosaccharides in the diet could effectively increase the expression of some anti-oxidation and immune related genes,and enhance the anti-oxidation level and immune ability of Exopalaemon carinicauda.The results of relative gene expression were consistent with the results of enzyme activity detection.In the challenge experiment,the survival rate of Exopalaemon carinicauda in T1,T2 and T3 treatments was significantly higher than that in CK(P<0.01)in 3-5 d.But the final survival rate of Exopalaemon carini-cauda in each treatment was the same as that in CK,which indicated that alginate oligosaccharides could prevent and con-trol Metschnikowia bicuspidate MQ2101,but could not improve the final survival rate after infection.
万方数据
维普
