Analysis of differentially expressed genes related to bolting in stem lettuce based on transcriptome data
To explore the key genes influencing bolting in stem lettuce,high-throughput transcriptome sequencing was employed to study the differentially expressed genes during the organ harvesting and bolting periods of stem lettuce.The results revealed that there were 6 754 differentially expressed genes in stem lettuce germplasm resource No.1 during the or-gan harvesting period and bolting period,while stem lettuce germplasm resource No.3 had 5 444 differentially expressed genes during these periods.Gene ontology(GO)functional enrichment analysis indicated that differentially expressed genes were mainly enriched in GO entries such as binding,catalytic activity,cellular processes,metabolic processes,and cellu-lar anatomical entities.Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis showed that stem lettuce germplasm resource No.1 and stem lettuce germplasm resource No.3 had 133 and 129 KEGG metabol-ic pathways respectively,with 128 metabolic pathways an-notated in both germplasm resources.KEGG metabolic pathways with a relatively higher enrichment of differential-ly expressed genes included biosynthesis of secondary metabolites,plant hormone signal transduction,and plant-pathogen interactions.Transcription factor analysis revealed that most of the differentially expressed genes during the organ harvesting and bolting periods of stem lettuce belonged to transcription factor families such as AP2/ERF,bHLH,bZIP,C2H2,MYB,NAC and WRKY.Comprehensive analysis results indicated that there were numerous differentially expressed genes in transcription factor families such as AP2/ERF,WRKY,bHLH,as well as pathways involving biosynthesis of secondary metabolites and plant hormone signal transduction.It was speculated that these transcription factor families and metabolic pathways could participate in the regulatory network of bolting in stem lettuce.These results can provide scientific basis for understanding the genes related to bolting in stem lettuce and their molecular mechanisms.
万方数据
维普
