Expression of bacteriophage lyase gene Lys BT1 in Chlorella vulgaris
Bacteriophage lyase has become a potential antibacterial drug due to its high efficiency and safe bacteri-cidal properties,and has been widely concerned.In this study,based on the preparation of high-efficiency bacteriophage lyase Lys BT1 by gene expression in the early stage,we deeply analyzed the structural characteristics of Lys BT1 interacting with the lyase receptor on bacteria,and successfully constructed the plasmid pCAMBIA 1301-BT1.The plasmid was intro-duced into Chlorella vulgaris by electrotransformation.The electrotransformation conditions were as follows:electric field in-tensity of 1.5 kV,pulse distance of 2 mm,and pulse time of 0.2 ms.After transient expression,the activity determination was successfully carried out.Therefore,the feasibility of Chlorella vulgaris as a lyase expression vector was verified,which provided a feasible path for the development of bacteriophage lyase expression system.
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