Distribution and resistant effects of blast resistant genes(Pi2,Pita,Pib)in japonica rice in Taihu area of Jiangsu province
In order to analyze the role of rice blast resistance genes Pi2,Pita and Pib in japonica rice blast resist-ance breeding in areas of Taihu area of Jiangsu province,penta-primer amplification refractory mutation system(PARMS)detecting technology was used to detect the distribution of rice blast resistance genes Pi2,Pita and Pib in 799 high genera-tion stable test materials screened and bred by Jiangsu Taihu Area Institute of Agricultural Sciences,and to analyze the re-lationship between genotype and resistance in combination with the results of artificial inoculation and identification of seed-ling blast and neck panicle blast.The results showed that among the 799 test materials,most of them carried two resistance genes,the distribution frequency of resistance gene Pib was the highest,the distribution frequency of resistance gene Pita was high,and the distribution frequency of resistance gene Pi2 was low.In the resistance gene combination,the detection rate of Pita+Pib combination was relatively high,which was 44.81%(358/799).The results of field dis-ease resistance identification showed that when there was only one single disease resistance gene in the test materi-als,materials with medium resistance or above accounted for 33.60%(86/256).When there were composite genotypes in the test materials,materials with medium resistance or above accounted for 61.02%(299/490),indicating that the coexis-tence of resistance genes could effectively enhance the resistance to rice blast.The results provide theoretical support for the selection of japonica rice blast resistance gene polymerization breeding in the Taihu region of Jiangsu province.
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