江苏太湖地区粳稻稻瘟病抗性基因(Pi2、Pita、Pib)的分布及抗病效应
Distribution and resistant effects of blast resistant genes(Pi2,Pita,Pib)in japonica rice in Taihu area of Jiangsu province
于雅洁 1曹鹏辉 1宋云生 1陈飞 1乔中英 1董明辉 1朱勇良 1谢裕林 1袁彩勇1
作者信息
- 1. 江苏太湖地区农业科学研究所,江苏 苏州 215104
- 折叠
摘要
为分析稻瘟病抗性基因Pi2、Pita、Pib在江苏太湖地区粳稻稻瘟病抗性育种中的作用,利用五引物扩增受阻突变体系(PARMS)检测技术,检测水稻稻瘟病抗性基因Pi2、Pita、Pib在江苏太湖地区农业科学研究所筛选培育的 799 份高世代稳定试验材料中的分布情况,并结合穗颈瘟人工接种鉴定结果分析基因型与抗性的关系.结果表明,在 799 份供试水稻品种中,以抗性基因 Pib的分布频率最高,抗性基因 Pita的分布频率也相对较高,抗性基因 Pi2 分布频率较低,多数材料携带 2 个抗性基因;在抗性基因组合中,Pita+Pib组合检出率较高,为 44.81%(358/799).田间抗病性鉴定结果表明,当供试材料中只存在单一抗病基因时,中抗级别以上材料占比为 33.60%(86/256),当供试材料中存在复合基因型时,中抗级别以上材料占比为 61.02%(299/490),表明抗性基因共存可以有效增强植株对稻瘟病的抗性.本研究结果为江苏太湖地区粳稻稻瘟病抗性基因聚合育种的选择提供了理论支持.
Abstract
In order to analyze the role of rice blast resistance genes Pi2,Pita and Pib in japonica rice blast resist-ance breeding in areas of Taihu area of Jiangsu province,penta-primer amplification refractory mutation system(PARMS)detecting technology was used to detect the distribution of rice blast resistance genes Pi2,Pita and Pib in 799 high genera-tion stable test materials screened and bred by Jiangsu Taihu Area Institute of Agricultural Sciences,and to analyze the re-lationship between genotype and resistance in combination with the results of artificial inoculation and identification of seed-ling blast and neck panicle blast.The results showed that among the 799 test materials,most of them carried two resistance genes,the distribution frequency of resistance gene Pib was the highest,the distribution frequency of resistance gene Pita was high,and the distribution frequency of resistance gene Pi2 was low.In the resistance gene combination,the detection rate of Pita+Pib combination was relatively high,which was 44.81%(358/799).The results of field dis-ease resistance identification showed that when there was only one single disease resistance gene in the test materi-als,materials with medium resistance or above accounted for 33.60%(86/256).When there were composite genotypes in the test materials,materials with medium resistance or above accounted for 61.02%(299/490),indicating that the coexis-tence of resistance genes could effectively enhance the resistance to rice blast.The results provide theoretical support for the selection of japonica rice blast resistance gene polymerization breeding in the Taihu region of Jiangsu province.
关键词
稻瘟病/PARMS技术/抗性基因/抗病育种Key words
rice blast/penta-primer amplification refractory mutation system(PARMS)technology/resistance genes/disease-resistance breeding引用本文复制引用
基金项目
江苏省"333"高层次人才培养项目()
苏州市科技计划项目(SNG2022012)
江苏太湖地区农业科学研究所院基金项目(21008)
出版年
2024
国家科技期刊平台
NSTL
万方数据