Analysis of genetic diversity and associated loci of grain shape traits in summer soybean regional test lines in Huang-Huai-Hai region based on SSR markers
To guide the parent selection and variety breeding of summer soybean in Huang-Huai-Hai region,the genotyping test of 192 summer soybean lines in Huang-Huai-Hai region was carried out by 135 pairs of SSR mark-ers distributed evenly on 20 chromosomes.The genetic dif-ferentiation and genetic diversity of the lines were ana-lyzed,the grain length,grain width and grain length-width ratio of the lines were measured,and the association anal-ysis of grain shape trait loci was carried out.The results showed that 365 alleles were detected by 135 pairs of markers,and an average of 2.703 7 alleles were detected by one marker,ranging from one to five.The polymorphic information content was 0-0.676 7,with an average of 0.368 6.The genetic diversity of 192 soybean lines(percentage of polymorphism=97.78%,number of alleles=2.703 7,effective number of alleles=1.956 1,Shannon's information index=0.709 0)was rich.According to the region of the tested soybean lines,they were divided into five natural populations.The genetic simi-larity(GI≥0.925 2)among natural populations was higher,and the genetic distance(GD≤0.077 8)was smaller,indi-cating that there were frequent exchanges among varieties and abundant genetic resources in Huang-Huai-Hai region.The results of population structure analysis and linkage disequilibrium analysis showed that 192 summer soybean lines were di-vided into five genetic subgroups,and there were different degrees of linkage disequilibrium in each genetic subgroup.The results of principal coordinate analysis showed that the first principal component,the second principal component and the third principal component explained 7.69%,6.23%and 5.57%of the total variation,respectively.The results of associa-tion analysis showed that 88 loci associated with grain shape traits were detected at the significant level of P＜0.05.Four lo-ci associated with grain shape traits were detected in two environments at the same time,which were located on chromo-somes 5,16 and 19,respectively.Among them,the Sat_071 locus on chromosome 19 had the highest phenotypic contribu-tion rate.
soybeangenetic diversitygrain shapeassociated loci
NETL
NSTL
万方数据
