Comparative analysis of SSR loci information of three Valeriana officinalis L.species based on transcriptome
To study the differences in SSR molecular markers of three Valeriana officinalis L.species,the corre-sponding primers were developed for the identification and molecular breeding of Valeriana officinalis L.species.In this study,Excel and MISA software were used to analyze the SSR loci data in the transcriptome of three Valeriana officinalis L.species.Primer 3.0 software was used to randomly design SSR primers,and PCR amplification and agarose gel electrophoresis were performed to verify the effectiveness of the primers and the polymorphism of SSR.The results showed that the transcriptome data of Valeriana officinalis L.(VOL),Valeriana officinalis var.iatifolia Miq.(VOI),and Valeriana officinalis var.officinalis Linn.(VOO)contained 29 462,40 840,and 29 280 Unigenes.These Unigenes included 7 515,8 046,and 7 330 SSR loci,respectively.The occurrence frequency of SSR loci in the three Valeriana officinalis L.species was 25.50%,19.71%,and 25.05%,with average distance of 4.16 kb,4.20 kb,and 4.22 kb.The predominant type of single-nucleotide repeat units in SSR was A/T,accounting for 41.76%,41.30%,38.50%of the total SSR.The number of unique repeat units of VOL,VOI,VOO was 44,45 and 25,respectively.The effective rate of SSR amplification by randomly designed primers was 77.78%,and the proportion of highly polymorphic SSR primers was 55.56%.In conclusion,the SSR loci of three Valeriana officinalis L.species exhibited characteristics such as high distribution density,high occurrence frequency,and diverse types of repeat units,indicating good polymorphism.The results of this study can provide a theoretical basis for the subse-quent identification and molecular breeding of the three Valeriana officinalis L.species.
NETL
NSTL
万方数据
