首页|油桐HSP70基因家族的全基因组鉴定与表达分析

油桐HSP70基因家族的全基因组鉴定与表达分析

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为探究油桐HSP70 基因的功能和进化关系,本研究对HSP70 基因家族成员进行了生物信息学鉴定和分析,包括理化性质、进化关系、基因结构、保守基序及染色体定位.同时分析了HSP70 基因家族成员在不同组织中的表达差异.结果表明,油桐 HSP70 基因家族包含 24 个家族成员,24 个 HSP70 蛋白的理论等电点为4.57～8.70,且大部分属于稳定的、亲水性蛋白质,系统进化树分析结果表明HSP70 家族成员分为 5 个聚类,油桐与毛果杨亲缘关系较近.24 个VfHSP70 蛋白氨基酸序列都包含HSP70 保守结构域.22 条HSP70 基因分布在 10 条染色体上.分析不同时空的VfHSP70 基因的表达量发现,VfHSP70-5在开花前 30 d、开花前 25 d、开花前 10d的花蕾中表达量均较高.VfHSP70-4在油桐根、茎、叶和种子中均有较高表达量.以上结果表明,VfHSP70 基因可能在调控油桐生长发育中具有重要作用.
Genome-wide identification and expression analysis of HSP70 gene family in Vernicia fordii
In order to investigate the function and evolution of HSP70 gene in Vernicia fordii,the members of HSP70 gene family were identified and analyzed by bioinformatics,including physical and chemical properties,evolutionary rela-tionships,gene structure,conserved motifs and chromosomal localization.The expression differences of HSP70 gene family members in different tissues were also analyzed.The results showed that the HSP70 gene family of Vernicia fordii contained 24 members,and the theoretical isoelectric points of 24 HSP70 proteins ranged from 4.57 to 8.70.Most of HSP70 proteins were stable and hydrophilic proteins.Phylogenetic tree analysis showed that HSP70 family members were di-vided into five clusters,and Vernicia fordii was closely re-lated to Populus trichocarpa.The amino acid sequences of 24 VfHSP70 proteins contained the conserved domain of HSP70.Twenty-two HSP70 genes were distributed on 10 chromosomes.By analyzing the expression of VfHSP70 gene in different time and space,it was found that the expression levels of VfHSP70-5 were higher in buds at 30 d before anthesis,25 d before anthesis and 10 d before anthesis.VfHSP70-4 was highly expressed in roots,stems,leaves and seeds of Vernicia fordii.These results suggest that VfHSP70 gene may play an important role in regulating the growth and develop-ment of Vernicia fordii.

Vernicia fordiiHSP70gene familybioinformatics

王占军、汪虹妍、杨妍萍、叶青芳、王淑雯、王朝霞、张翼、张勰

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合肥师范学院生物与食品工程学院,安徽 合肥 230601

湖南省林业科学院省部共建木本油料资源利用国家重点实验室,湖南 长沙 410004

油桐 HSP70 基因家族 生物信息学

省部共建木本油料资源利用国家重点实验室开放课题省部共建木本油料资源利用国家重点实验室开放课题安徽省高校优秀青年人才支持计划项目安徽省级大学生创新创业训练计划项目安徽省级大学生创新创业训练计划项目国家级大学生创新创业训练计划项目

GZKF202103GZKF202203gxyq2020040S202414098204S202414098205202414098077

2024

10.3969/j.issn.1000-4440.2024.05.005

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子：1.093
ISSN：1000-4440
年,卷(期)：2024.40(5)
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