Remediation of chlorpyrifos contaminated soil by biochar immobilized bac-teria and improvement of Chinese cabbage quality
Biochar can adsorb organic pollutants in the soil and reduce the phytotoxicity of pollutants,but the adsorbed pollutants have the risk of secondary release,which affects the remediation performance of biochar.In this study,biochar was used to immobilize three endophytes with chlorpyrifos degradation function selected in the early laboratory stage.The prepara-tion conditions of biochar immobilized bacteria were optimized,and the degradation performance of chlorpyrifos by biochar im-mobilized bacteria was verified by pot experiment.The results showed that HJY had the best degradation effect on chlorpyrifos.The biochar immobilized microbial agent was prepared under the conditions of biochar particle size of 60 mesh,immobilization temperature of 30℃and immobilization time of 1 d.The degradation rate of chlorpyrifos was 92.40%when the prepared biochar immobilized microbial agent was applied to the soil for 15 days.After 35 days of application to the soil,in the 2 mg/kg chlorpyrifos contaminated soil,compared with biochar treatment,the content of chlorpyrifos in Chi-nese cabbage and soil in the biochar immobilized microbial agent treatment decreased by 81.8%and 77.4%,respectively.In 20 mg/kg chlorpyrifos contaminated soil,the content of chlorpyrifos in Chinese cabbage and soil decreased by 79.4%and 50.3%,respectively.The biomass,chlorophyll content,soluble protein content,soluble sugar content,peroxidase(POD)activity and superoxide dismutase(SOD)activity of Chinese cabbage increased significantly.There was a significant positive correlation between the content of chlorpyrifos in Chinese cabbage and the content of chlorpyrifos in soil,and there was a sig-nificant negative correlation between the quality of Chinese cabbage and the content of chlorpyrifos.Compared with biochar,biochar-immobilized microbial agent significantly improved the remediation performance of chlorpyrifos-contaminated soil and improved the quality of Chinese cabbage.
NETL
NSTL
万方数据
