Exploration of genes related to the naked neck trait in Tianjin-monkey chickens based on WGCNA
Tianjin-monkey chicken is a precious genetic resource of naked neck chicken in China.To identify genes related to the naked neck phenotype at the whole-genome level,we collected neck skin tissue from the hybrid F1 generation of Tianjin-monkey chickens for transcriptome sequencing.The results showed that among the 10 gene modules identified by weighted gene co-expression network analysis(WGCNA),only the turquoise and blue modules were significantly associated with the naked neck phenotype,comprising a total of 583 genes.GO enrichment analysis revealed biological process terms related to fat and muscle,including lipid droplet organization,negative regulation of triglyceride storage and muscle con-traction.KEGG pathway analysis also identified multiple pathways related to fat metabolism,such as PPAR signaling path-way and glycerolipid metabolism.Protein-protein interaction network analysis revealed that the protein encoded by ACTN2 had the highest connectivity,followed by the protein encoded by MYL10.Furthermore,several genes closely involved in hair follicle development,such as SOX9 and PPARGC,were also found.The results of this study can lay a foundation for further revealing and improving the forma-tion and development of naked neck in chickens.Moreo-ver,it is of great significance for the protection,develop-ment,and utilization of Tianjin-monkey chicken.
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