Effects of tail tip removal treatments on the content of hormones in the basal buds of chestnut fruiting branches
Analyzing the effects of tail tip removal treatments on the content of hormones in basal buds can provide a certain reference for understanding the germination conditions of hidden buds.In this study,Yanqiu was used as the experi-mental material.Tail tip removal treatments were performed on the first day(TA,May 25),21st day(TB,June 14)and 41st day(TC,July 4)after flowering.The basal buds were taken on the 5th,10th,15th,and 20th days after each removal of the tail tip.The contents and ratios of strigolactone(SL),gibberellin(GA),auxin,cytokinin(CTK)and abscisic acid(ABA)in the basal buds of chestnut fruiting branches after different treatments were determined by liquid chromatography tandem mass spectrometry(LC-MS/MS).The results showed that the average number of one-year-old branches germinated from the basal buds was promoted after the removal of tail tip of chestnut fruiting branches.TA treatment increased the ratio of fruiting branches(16.67%),branch length of developing branches and the diameter of fruiting branches.After TB treat-ment and TC treatment,only developing branches germinated from the basal buds.The basal buds of the control did not ger-minate,and only the top buds germinated one-year-old branches,with an average of three branches,and the ratio of fruit-ing branches was 20.00%.The SL content decreased greatly in TA treatment.The content of GA decreased greatly in TB treatment.The content of auxin decreased in TA treatment,and increased greatly in TB treatment.The fluctuation range of CTK content was similar to that of the control,and the difference was not significant.The ABA content increased greatly in TC treatment,but decreased in TA treatment.The ratio of indole-3-acetic acid(IAA)content to ABA content decreased o-verall,and it decreased greatly in TC treatment.The ratio of IAA content to GA content increased greatly in TC treatment,and the fluctuation was obvious.The ratio of ABA content to GA content decreased in TA treatment,and increased signifi-cantly in TC treatment.In addition,correlation analysis showed that there was a significant positive correlation between gib-berellin content and abscisic acid content in the control(r=0.646).The correlation between 29 hormones in the treatments was mainly concentrated between cytokinin and auxin.Among them,the content of IA(3-indoleacrylic acid)was signifi-cantly positively correlated with the contents of IAA-Ala[N-(3-indolylacetyl)-L-alanine],ICA(indole-3-carboxylic acid),TRA(tryptamine),cZROG(cis-zeatin O-glucoside riboside),ABA-GE(abscisic acid-glucosyl ester),DHZ7G(dihydrozeatin-7-glucoside),ICAld(indole-3-carboxaldehyde),tZOG(trans-zeatin-O-glucoside).The IPR(N6-isopen-tenyladenosine)content and tZR(trans-zeatin riboside)content were significantly negatively correlated with TRA content,ILA(indole-3-lactic acid)content and cZROG(cis-zeatin-O-glucoside riboside)content.In summary,the removal of tail tip of the chestnut could promote the germination of the basal buds,and the TA treatment could promote the germination of the fruiting branches from the basal axillary buds.After the removal of the tail tip,the content of GA decreased as a whole,and the low level of GA was helpful to promote the growth of axillary buds.The low level of ABA content was helpful for the germination of fruiting branches from basal buds.High levels of the ratio of ABA content to GA content could inhibit the germination of fruiting branches from axillary buds.The low level of IAA content in a reasonable range may promote the ger-mination of chestnut fruiting branches from basal buds.
chestnuttail tip treatmentbasal budliquid chromatography tandem mass spectrometryhormone
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