北苑贡茶茶树种质遗传多样性及其与青心乌龙茶树的亲缘关系
Genetic diversity of Beiyuan tribute tea germplasms and their genetic rela-tionship with Qingxinwulong
刘财国 1吕水源 2于文涛 2潘宏英 3王攀 1严林华 3朱艳宇 1金珊 1叶乃兴1
作者信息
- 1. 福建农林大学园艺学院,福建 福州 350002
- 2. 福州海关技术中心,福建 福州 350001
- 3. 建瓯市茶业发展中心,福建 建瓯 353100
- 折叠
摘要
为更好地发掘、保护和利用北苑贡茶茶树种质资源,采用EST-SNP分子标记技术对北苑贡茶种质遗传多样性及其与青心乌龙的亲缘关系进行分析.筛选出 49 个多态性强的SNP位点,适用于鉴定北苑贡茶茶树种质基因分型以及分析其与青心乌龙的亲缘关系,并进行PCoA主成分分析、层级进化树构建和遗传距离分析.结果表明,北苑贡茶茶树种质具有丰富的遗传多样性,其 6 个茶树种质群体间的遗传距离为0.021～0.137;北苑贡茶核心产地福建省建瓯市东峰镇的百年乌龙茶园为有性茶树-无性茶树混合种植的古茶园;青心乌龙茶树(中国台湾)、矮脚乌龙茶树(武夷山)与北苑贡茶的百年乌龙茶树种质亲缘关系密切.根据亲缘关系分析推测,青心乌龙茶树(中国台湾)、软枝乌龙茶树(中国台湾)和矮脚乌龙茶树(武夷山)可能均源自建瓯北苑贡茶产区.
Abstract
To enhance the excavation,protection and utilization of excellent Beiyuan tribute tea germplasm re-sources,the genetic diversity of Beiyuan tribute tea germplasms and their genetic relationship with Qingxinwulong were ana-lyzed using the EST-SNP molecular marker technique.Forty-nine SNP loci with strong polymorphism were screened out,which were suitable for identifying the genotyping of Beiyuan tribute tea germplasm and analyzing its genetic relationship with Qingxinwulong,and the PCoA principal component analysis,hierarchical phylogenetic tree construction and genetic distance analysis were carried out.The results showed that the germplasm resources of Beiyuan tribute tea had abundant ge-netic diversity,and the genetic distance among the six tea groups ranged from 0.021 to 0.137.The century-old oolong tea garden in Dongfeng Town,the core producing area of Beiyuan tribute tea,was an ancient tea garden with mixed planting of sexual and asexual.Qingxinwulong(Tai-wan)and Aijiaowulong(Wuyishan)were closely related to the centennial oolong germplasm of Beiyuan tribute tea.According to the analysis of genetic relationship,it was speculated that Qingxinwulong(Taiwan),Ruanzhiwulong(Taiwan)and Aijiaowulong(Wuyishan)might be derived from Jian'ou Beiyuan tribute tea producing area.
关键词
北苑贡茶/青心乌龙/茶树/种质资源/遗传多样性Key words
Beiyuan tribute tea/Qingxinwulong/Camellia sinensis/germplasm resources/genetic diversity引用本文复制引用
基金项目
建瓯市茶叶科技创新专项(JO2022001)
福建省科技厅农业科技引导性项目(2021N0024)
福建农林大学科技创新专项基金项目(CXZX2020123B)
海关总署科研项目(2020HK187)
出版年
2024
国家科技期刊平台
NSTL
万方数据