Identification of adzuki bean(Vigna angularis)germplasm resources based on SNP molecular markers and phenotypic traits
In order to establish a scientific and reliable method for identification of adzuki bean[Vigna angularis(Willd)Ohwi&H.Ohashi]germplasm resources,102 adzuki bean germplasm resources were clustered and fingerprinted by sin-gle nucleotide polymorphism(SNP)molecular markers,at the same time,the germplasm resources of adzuki bean were identi-fied combined with qualitative traits.The results showed that 26 SNP molecular markers in 102 adzuki bean germplasm resources showed polymorphism,and the average polymorphism information content was 0.28.The SNP fingerprints of 61 adzuki bean germ-plasm resources were unique and could be accurately identified.The SNP fingerprints of the remaining 41 adzuki bean germplasm resources were not unique.The average genetic diversity index of six qualitative traits was 1.224 5 in 102 germplasm resources through further analysis of genetic variation,and all of the six traits were highly polymorphic.Combined with the above six traits,germplasm resources with the same SNP fingerprints can be further distinguished.The identification method of SNP molecular markers combined with phenotypic traits developed in this study can lay a foundation for genetic identification and scientific utilization of adzuki bean germplasm resources.
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