首页|油桐ARF基因家族的鉴定及表达分析

油桐ARF基因家族的鉴定及表达分析

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
植物生长发育由多种激素共同调控,其中生长素是重要的调控激素之一.生长素响应因子(Auxin response factor,ARF)是生长素信号传导过程中的关键因子,它能够特异性地与生长素应答元件结合,进而影响植物体内生长素响应基因的表达.本研究通过生物信息学方法鉴定到17个油桐ARF基因家族成员,分布在9条染色体上,并且各基因间无片段重复现象.理化性质分析结果表明,ARF蛋白长度为587～1 119 aa,均为酸性蛋白质,不稳定系数＞40,为不稳定蛋白质,亲水性指数＜0,为亲水性蛋白质.根据系统进化分析结果将油桐ARF蛋白家族分为4个亚族,与拟南芥的亚族划分一致.每个成员都具有典型的B3型结构域和Auxin_resp中间结构域.分析ARF基因表达特征发现,ARF基因的表达在油桐不同组织中具有明显的组织特异性.本研究结果为揭示油桐生长发育调控机制提供了理论依据.
Identification and expression analysis of ARF gene family in Vernicia fordii
Plant growth and development is regulated by many hormones,among which auxin is one of the important regulatory hormones.Auxin response factor(ARF)is a key factor in the process of auxin signal transduction,which can spe-cifically bind to auxin response elements and affect the expression of auxin response genes in plants.In this study,17 mem-bers of ARF gene family in Vernicia fordii were identified by bioinformatics method,which were distributed on nine chromo-somes,and there was no fragment duplication between genes.The results of physical and chemical properties analysis showed that the length of ARF proteins was 587-1 119 aa,all of them were acid proteins.The instability coefficients of ARF proteins were more than 40,and the ARF proteins were unstable proteins.The hydropathicity indexes of ARF proteins were less than 0,and the ARF proteins were hydrophilic proteins.According to the results of phylogenetic analysis,the ARF protein family of Vernicia fordii was divided into four subfamilies,which was consistent with the subfamily division of Arabidopsis thaliana.Each member had a typical B3 domain and an auxin_resp intermediate domain.By analyzing the expres-sion characteristics of ARF gene,it was found that the ex-pression of ARF gene had obvious tissue specificity in dif-ferent tissues of Vernicia fordii.The results of this study provide a theoretical basis for revealing the regulatory mech-anism of Vernicia fordii growth and development.

Vernicia fordiiARF gene familybioinformatics analysisexpression analysis

王占军、李静静、周子寒、孙婉、王毅、张勰

展开 >

合肥师范学院生物与食品工程学院,安徽 合肥 230601

湖南省林业科学院省部共建木本油料资源利用国家重点实验室,湖南 长沙 410004

油桐 ARF基因家族 生物信息学分析 表达分析

省部共建木本油料资源利用国家重点实验室开放课题省部共建木本油料资源利用国家重点实验室开放课题安徽省高校优秀青年人才支持计划项目安徽省级大学生创新创业训练计划项目安徽省级大学生创新创业训练计划项目国家级大学生创新创业训练计划项目2022年度引进高层次人才科研启动基金项目

GZKF202103GZKF202203gxyq2020040S202414098204S2024140982052024140980772022rcjj12

2024

10.3969/j.issn.1000-4440.2024.06.004

江苏农业学报
江苏省农业科学院

江苏农业学报

CSTPCD北大核心
影响因子：1.093
ISSN：1000-4440
年,卷(期)：2024.40(6)