Effects of root-knot nematode disease on microbial community and func-tion in rhizosphere soil of tobacco plants
In order to clarify the response characteristics of rhizosphere soil microorganisms of tobacco plants to its root-knot nematode disease,Illumina MiSeq high-throughput sequencing technology was used to determine the bacterial 16S rDNA and fungal internal transcribed spacer(ITS)sequences in the rhizosphere soil of healthy tobacco plants(FH-H)and diseased tobacco plants(FH-D).The physicochemical properties and enzyme activities of the rhizosphere soil were determined,and PICRUSt2 software and FUNGuild data-base analysis were performed.The results showed that com-pared with healthy tobacco plants,the pH,nitrate nitrogen content,alkali-hydrolyzable nitrogen content,catalase ac-tivity and microbial community α diversity of rhizosphere soil of diseased tobacco plants decreased significantly.The microbial species and relative abundance changed.The relative a-bundances of Actinobacteria,Firmicutes,Bacillus and Unclassified_Ktedonobacteraceae in the bacterial community increased significantly,and the relative abundances of Ascomycota,Basidiomycota,Penicillium and Trechispora in the fungal communi-ty increased significantly.The abundances of carbohydrate metabolism,amino acid metabolism,cofactors and vitamin metabo-lism of the bacterial community in the rhizosphere soil of diseased tobacco plants were significantly higher than those in the rhizosphere soil of healthy tobacco plants.The relative abundance of functional fungal types in the rhizosphere soil of diseased tobacco plants was obviously different from that of healthy tobacco plants.The relative abundance of saprophytic functional fungi in the rhizosphere soil of diseased tobacco plants was significantly higher than that of healthy tobacco plants.The results of variance decomposition analysis showed that the soil available phosphorus content had the greatest influence on the bacterial relative abundance in the rhizosphere soil of tobacco plants.The fungal relative abundance in the rhizosphere soil of tobacco plants was mainly correlated with soil urease activity,soil nitrate nitrogen content and soil pH.The results of this study pro-vide a theoretical basis for the green prevention and control of root-knot nematode disease and soil conservation.
root-knot nematodestobaccorhizosphere soilmicrobial communitymicrobial function
NETL
NSTL
万方数据
