Screening of SNP loci of MIH gene in Eriocheir sinensis and its association with growth traits
In order to clarify the regulatory mechanism of molt-inhibiting hormone(MIH)gene on the growth of Chi-nese mitten crab(Eriocheir sinensis),100 juvenile Chinese mitten crabs were selected to analyze the single nucleotide poly-morphism(SNP)loci and genotypes of MIH gene,and the linkage disequilibrium and haplotype analysis of polymorphic loci related to growth indicators were carried out to further clarify the correlation between haplotypes of polymorphic loci and growth traits.The results showed that five SNP loci were screened and identified in MIH gene of juvenile Chinese mitten crab,and three of them(C640G,C2529T and G2595T)were related to the growth traits of Chinese mitten crab.Five haplotypes were detected in the three related sites.Among them,H1 haplotype(GCG)accounted for the highest proportion(68.8%),which was the dominant haplotype.The growth traits of individuals with H3 haplotype(GTT)were the highest and significant-ly higher than those of individuals with H2 haplotype(CCG).The three SNP loci associated with growth traits in MIH gene of Chinese mitten crab obtained in this study can be used as candidate molecular markers for the breeding of high-quality Chinese mitten crab varieties.
NETL
NSTL
万方数据
