辣椒CaTPS11基因克隆及非生物胁迫下的表达
Cloning of CaTPS11 gene and expression under abiotic stress in pepper
马艳 1苟秉调 1赵淑芳 1魏敏 1段盼盼 1匡小妍 1张涛 1魏兵强1
作者信息
- 1. 甘肃农业大学园艺学院,甘肃 兰州 730070
- 折叠
摘要
本研究以强丰 101 辣椒品种为材料,分析辣椒CaTPS11 基因编码蛋白质的理化性质、蛋白质氨基酸序列及组织特异性表达,利用荧光定量PCR技术分析CaTPS11 基因在低温、激素处理过程中的表达量变化.结果表明,CaTPS11 基因全长2 805 bp,编码921 个氨基酸.与辣椒基因组数据库参考序列比对,CaTPS11 基因存在5 个单碱基差异和 2 处内含子保留剪切,启动子区存在植物激素、低温、光等多种响应元件.CaTPS11 蛋白相对分子量为1.033 9×105,理论等电点8.33;无跨膜结构和信号肽,具有TPS蛋白家族的典型结构域.亚细胞定位分析结果表明CaTPS11 蛋白定位于细胞质和叶绿体中.CaTPS11 蛋白氨基酸序列与龙葵、马铃薯TPS蛋白氨基酸序列同源关系最近且进化高度保守.CaTPS11 基因在成熟果胎座中的表达量最高,为成熟果果肉的 26 倍.IAA 处理抑制CaTPS11 基因的表达,低温胁迫、ABA、GA、MeJA处理均诱导该基因的表达.MeJA处理 6h,辣椒CaTPS11 基因表达量达到最大,约为对照的 9 倍.CaTPS11 基因存在时空表达差异,推测该基因通过内含子保留的可变剪切模式和MeJA信号通路正向响应非生物胁迫的方式提高自身表达,以应对逆境胁迫.
Abstract
In this study,the physicochemical properties of the protein encoded by CaTPS11 gene,amino acid sequence and tissue-specific expression were analyzed by using Qiangfeng 101 pepper as the material.The expression of CaTPS11 gene in low temperature and hormone treatments was analyzed by real-time quantitative PCR.The results showed that CaTPS11 gene was 2 805 bp in length and encoded 921 amino acids.Compared with the reference sequence of pepper genome database,the CaTPS11 gene had five single-base differences and two intron-retained splits,and many response elements such as plant hor-mone,low temperature and light were found in the promoter region.The relative molecular weight of CaTPS11 protein was 1.033 9×105,with theoretical isoelectric point of 8.33.CaTPS11 protein had no transmembrane structure and signal peptide,and it had the typical domain of TPS protein family.Subcellular localization analysis indicated that CaTPS11 protein was localized in cytoplasm and chloroplast.The amino acid sequence of CaTPS11 protein was closely related to the amino acid sequence of TPS protein of Solanum nigrum L.and potato,and the evolution was highly conserved.The expression level of CaTPS11 gene in mature fruit placenta was the highest,and was 26 times higher than that in mature fruit pulp.IAA treatment inhibited the expression of CaTPS11 gene,while cold stress treatment,ABA treatment,GA treatment and MeJA treatment induced the expression of CaTPS11 gene.The expres-sion level of CaTPS11gene in pepper treated with MeJA for 6 h was nine times higher than that in the control.The CaTPS11gene had spatial and temporal expression differences.It was inferred that CaTPS11 gene could enhance its expression in response to abiotic stress by means of variable splicing mode of intron retention and positive response of MeJA signal pathway.
关键词
辣椒/CaTPS11基因/非生物胁迫/基因表达Key words
pepper/CaTPS11 gene/abiotic stress/gene expression引用本文复制引用
基金项目
国家自然科学基金项目(31760572)
甘肃省重点研发计划项目(21YF5NA091)
兰州市人才创新项目(2021-RC-65)
甘肃农业大学青年导师扶持项目(GAU-QDFC-2020-07)
出版年
2024
国家科技期刊平台
NSTL
万方数据