Cloning of CaTPS11 gene and expression under abiotic stress in pepper
In this study,the physicochemical properties of the protein encoded by CaTPS11 gene,amino acid sequence and tissue-specific expression were analyzed by using Qiangfeng 101 pepper as the material.The expression of CaTPS11 gene in low temperature and hormone treatments was analyzed by real-time quantitative PCR.The results showed that CaTPS11 gene was 2 805 bp in length and encoded 921 amino acids.Compared with the reference sequence of pepper genome database,the CaTPS11 gene had five single-base differences and two intron-retained splits,and many response elements such as plant hor-mone,low temperature and light were found in the promoter region.The relative molecular weight of CaTPS11 protein was 1.033 9×105,with theoretical isoelectric point of 8.33.CaTPS11 protein had no transmembrane structure and signal peptide,and it had the typical domain of TPS protein family.Subcellular localization analysis indicated that CaTPS11 protein was localized in cytoplasm and chloroplast.The amino acid sequence of CaTPS11 protein was closely related to the amino acid sequence of TPS protein of Solanum nigrum L.and potato,and the evolution was highly conserved.The expression level of CaTPS11 gene in mature fruit placenta was the highest,and was 26 times higher than that in mature fruit pulp.IAA treatment inhibited the expression of CaTPS11 gene,while cold stress treatment,ABA treatment,GA treatment and MeJA treatment induced the expression of CaTPS11 gene.The expres-sion level of CaTPS11gene in pepper treated with MeJA for 6 h was nine times higher than that in the control.The CaTPS11gene had spatial and temporal expression differences.It was inferred that CaTPS11 gene could enhance its expression in response to abiotic stress by means of variable splicing mode of intron retention and positive response of MeJA signal pathway.
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