二氢卟吩铁调控辣椒对高温胁迫的耐性及相关基因的表达
Regulation of iron chlorine e6 on high temperature stress tolerance and re-lated gene expression in pepper
李萌楠 1乐秀虎 2周阳 3季英华 4魏利辉 4周冬梅 4谢加飞5
作者信息
- 1. 河北工程大学园林与生态工程学院,河北 邯郸 471023;江苏省农业科学院植物保护研究所,江苏 南京 210014
- 2. 河北工程大学园林与生态工程学院,河北 邯郸 471023
- 3. 全国农业技术推广服务中心,北京 100125
- 4. 江苏省农业科学院植物保护研究所,江苏 南京 210014
- 5. 高邮市农业农村局,江苏 高邮 225600
- 折叠
摘要
为评估高温胁迫下二氢卟吩铁(Iron chlorine e6,ICE6)缓解辣椒幼苗高温伤害效应及对耐高温胁迫相关基因表达量的影响.本研究以艳椒465为供试品种,探究高温胁迫下,二氢卟吩铁对辣椒幼苗相对电导率、可溶性糖含量、抗氧化酶活性、脯氨酸含量等生理生化指标和耐高温相关基因表达的影响.试验结果表明,高温胁迫下,与清水对照比,喷施0.2 μg/ml二氢卟吩铁可提高辣椒叶片中可溶性糖、脯氨酸、还原型谷胱甘肽含量,增强超氧化物歧化酶、过氧化物酶活性,降低相对电导率及丙二醛含量;二氢卟吩铁能提高CaWRKY19、CaWRKY55 和CaWRKY40 耐高温胁迫相关基因的表达.由此可见,用0.2 μg/ml二氢卟吩铁处理可显著提高辣椒耐高温胁迫能力.
Abstract
The purpose of this study was to evaluate the effects of iron chlorine e6(ICE6)on alleviating high tem-perature damage and the expression of heat tolerance-related genes in pepper seedlings under high temperature stress.In this study,Yanjiao 465 was used as the test variety to explore the effects of iron chlorine e6 on physiological and biochemi-cal indices such as relative conductivity,soluble sugar content,antioxidant enzyme activity,proline content and the expres-sion of heat tolerance-related genes in pepper seedlings under high temperature stress.The results showed that under high temperature stress,compared with the control,spraying 0.2 μg/ml iron chlorine e6 could increase the contents of soluble sugar,proline and reduced glutathione in pepper leaves,enhance the activities of superoxide dismutase and peroxidase,and reduce the relative conductivity and malondialdehyde content.Iron chlorine e6 could increase the expression of heat tolerance-related genes CaWRKY19,CaWRKY55 and CaWRKY40.Therefore,the treatment with 0.2 μg/ml iron chlorine e6 could significantly improve the high tempera-ture resistance of pepper.
关键词
辣椒/二氢卟吩铁/高温胁迫/耐高温胁迫基因Key words
pepper/iron chlorine e6/high-tem-perature stress/heat tolerance-related genes引用本文复制引用
基金项目
江苏省农业科技自主创新基金项目(CX231033)
河北省自然科学基金项目(C2019402344)
出版年
2024
国家科技期刊平台
NSTL
万方数据