Regulation of iron chlorine e6 on high temperature stress tolerance and re-lated gene expression in pepper
The purpose of this study was to evaluate the effects of iron chlorine e6(ICE6)on alleviating high tem-perature damage and the expression of heat tolerance-related genes in pepper seedlings under high temperature stress.In this study,Yanjiao 465 was used as the test variety to explore the effects of iron chlorine e6 on physiological and biochemi-cal indices such as relative conductivity,soluble sugar content,antioxidant enzyme activity,proline content and the expres-sion of heat tolerance-related genes in pepper seedlings under high temperature stress.The results showed that under high temperature stress,compared with the control,spraying 0.2 μg/ml iron chlorine e6 could increase the contents of soluble sugar,proline and reduced glutathione in pepper leaves,enhance the activities of superoxide dismutase and peroxidase,and reduce the relative conductivity and malondialdehyde content.Iron chlorine e6 could increase the expression of heat tolerance-related genes CaWRKY19,CaWRKY55 and CaWRKY40.Therefore,the treatment with 0.2 μg/ml iron chlorine e6 could significantly improve the high tempera-ture resistance of pepper.
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