Screening and mapping of genes related to recessive nuclear male sterility in watermelon
To explore more genes related to watermelon anther and pollen development and analyze its regulatory net-work,the genetic segregation population was constructed using watermelon male sterile materials and male fertile materials.And the gene mapping and fertility related gene screening were carried out by using BSA-seq and RNA-seq.The results showed that male sterility trait was controlled by a single recessive nuclear gene,which was mapped between 5 766 829 bp and 21 366 400 bp on chromosome 6.In combination with SNP/InDel variation site,gene annotation and expression analy-sis,one key candidate differentially expressed gene Cla97C06G117840 and 20 other differentially expressed genes affected by the expression of this gene were obtained.Bioinformatics analysis suggested that the deletion of some coding sequences of Cla97C06G117840 led to premature termination of the coding protein,resulting in pollen abortion.In addition,20 other differentially expressed genes were enriched in the gene pathway of anther wall tapetum development and pollen wall assem-bly.These genes might form a gene regulatory network to affect anther and pollen development.This study can lay a founda-tion for later gene function verification.
watermelonmale sterilityfertility related genesgene mapping
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