Phenotypic traits analysis and establishment of molecular identity card of Xinjiang pomegranate
In order to quickly identify pomegranate germplasm resources,solve the phenomenon of homonyms and synonyms,and promote the development of pomegranate industry in Xinjiang,this study collected 24 germplasms from the main producing areas of pomegranate in Xinjiang,and analyzed the variation characteristics of phenotypic traits of leaves and fruits of pomegranate in Xinjiang.Eighteen primers with good polymorphism were selected to analyze the genetic diversi-ty and genetic relationship of pomegranate germplasms,and the core primers that could identify 24 pomegranate germplasms were selected to construct the molecular identity card of pomegranate in Xinjiang.The results showed that the shape of fruits and leaves of pomegranate in Xinjiang varied greatly,and the genetic diversity was rich,but it was difficult to accurately identify pomegranate germplasms by phenotypic traits.A total of 240 bands were amplified by 18 primers,of which 199 were polymorphic bands,accounting for 82.92%.The average number of alleles of 24 germplasms was 1.812 5,the effective number of alleles was 1.287 0,Nei's gene diversity index was 0.177 0,and the Shannon's diversity index was 0.282 0.The Nei's gene diversity index and Shannon's diversity index of pomegranate germplasms in Hotan prefecture were higher than those in Kashi prefecture.When the genetic similarity coefficient was 0.85,24 pomegranate germplasms were divided into six groups,and the classifi-cation results of pomegranate were not related to fruit flavor and geographical location.Five core primers(UBC73,UBC880,UBC810,UBC841 and UBC35)could be used to identify 24 pomegranate germplasms.The molecular identity cards of Xinjiang pomegranate constructed by the amplified electrophoresis patterns were also different,indicating that there was no phenomenon of homonyms and synonyms in 24 germplasms.The results of this study provide a new method for the i-dentification of pomegranate germplasms.
NETL
NSTL
万方数据
