Differences in ALS inhibitor herbicide resistance among rice mutants with different loci induced by EMS mutagenesis
Weeds in paddy fields seriously threaten the growth of rice.Breeding new varieties of herbicide-resistant rice is one of the important ways to effectively solve the harm of weeds in paddy fields.In this study,ethyl methane sulfonate(EMS)was used as a chemical mutagen to induce Huazhan rice to create mutants.The mutants were screened by the acetolactate syn-thase(ALS)inhibitor herbicide imazapic(20 g/hm2)to obtain resistant mutant materials.The OsALS gene sequences of the a-bove mutants were analyzed to clarify the mutation type.Finally,the cross-resistance of different mutant types to imazamox and mesosulfuron-methyl was analyzed.The results showed that 11 imazapic-resistant mutants were obtained from Huazhan rice by EMS mutagenesis and imazapic screening.The mutation types were Pro171Leu,Ser627Cys,Ser627Asn and Gly628Glu.Among them,Ser627Cys mutation type was first reported.The resistance levels of the four mutation types to imazapic were different.The resistance indices of Ser627Asn,Ser627Cys,Pro171Leu and Gly628Glu were 8.71,5.16,3.28 and 3.69,respectively.There were also significant differences in the resistance of the four mutation types to imazamox and mesosulfuron-methyl.The resistance of Ser627Asn to imazamox was the highest,and the resistance index was 20.57.The resistance of Gly628Glu to mesosulfuron-methyl was the highest,and the resist-ance index was 5.11.In summary,four Huazhan rice mutant types resistant to ALS inhibitor herbicides were obtained by EMS mu-tagenesis,and their resistance differences to imazapic and the cross-resistance to imazamox and mesosulfuron-methyl were identi-fied,which provided a basis for further cultivation of herbicide-resistant rice varieties and rotation of herbicides.
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维普
