Identification of BnWSD family genes in Brassica napus L.and their ex-pression characteristics under drought stress
Drought stress can induce the expression of crop wax synthase/diacylglycerol acyltransferase(WSD)gene.In order to clarify the physical and chemical properties of BnWSD family genes in Brassica napus and their response characteristics to drought stress,this study used bioinformat-ics methods to screen BnWSD family genes from the B.napus genome and analyzed their structure,chromosome location,phylogenetic tree and the physical and chemical properties,structure,conserved motifs and cis-acting elements of their encoding proteins,based on the B.napus transcriptome database.The expression characteristics of BnWSD family genes were de-termined by drought stress treatment with Longyou 10 as the experimental material.The results showed that a total of 36 BnWSD family genes were identified in the genome of B.napus,of which could be divided into three subfamilies.The number of amino acids of encoded proteins of 36 BnWSD family genes ranged from 105 to 538,and the isoelectric point ranged from 6.32 to 9.63.There were eight conserved motifs in 36 BnWSD family genes,and each gene contained two to eight conserved motifs,and motif 2 was a common functional motif in the BnWSD family genes,which was relatively conserved in evolution.There were 11 cis-act-ing elements related to light response,hormone response and drought stress response in the promoter region of BnWSD gene in B.napus.The relative expression levels of BnWSD gene in different organs of B.napus were different.The relative expression lev-els in leaves and stems were relatively higher,while the relative expression levels in roots and flowers were relatively lower or not expressed.With the deepening of drought,the relative expression levels of BnWSD1-1,BnWSD2,BnWSD8,BnWSD9,Bn-WSD12,BnWSD18-1,BnWSD20-1 and BnWSD24-1 in leaves of B.napus showed an increasing trend.In summary,the BnWSD gene was closely related to the drought resistance of B.napus.The results of this study provide a basis for further analysis of the drought resistance mechanism of B.napus and clarifying the role of the BnWSD gene family in epidermal wax accumulation and the breeding of drought-resistant B.napus varieties.
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