Soybean transcription factor GmMYC2L is involved in the regulation of plant salt tolerance
Transcription factors play an important role in the response and regulation of plants to adversity stress.In order to explore the function of soybean bHLH transcription factor family member GmMYC2L in plant salt tolerance,the conserved domains of soybean GmMYC2L protein and MYC2 proteins of sweet potato,tomato,sesame,Nicotiana benthami-ana and Arabidopsis thaliana were analyzed by sequence homology comparison.The expression pattern of soybean Gm-MYC2L gene induced by salt stress was detected by semi-quantitative PCR.The subcellular localization of GmMYC2L pro-tein was analyzed by transient expression in tobacco leaves.GmMYC2L gene transgenic Arabidopsis thaliana plants were ob-tained by floral dip method.The response and mechanism of wild-type and transgenic Arabidopsis thaliana plants to salt stress were analyzed.The results showed that soybean GmMYC2L protein had a typical conserved domain of the bHLH fami-ly and the protein was localized in the nucleus.Under salt stress,the expression of GmMYC2L gene in soybean roots and leaves was significantly up-regulated.Under salt stress,the contents of malondialdehyde and hydrogen peroxide in the leav-es of GmMYC2L overexpressing Arabidopsis thaliana plants were significantly lower than those of wild-type Arabidopsis thali-ana plants,and the superoxide dismutase(SOD)activity and peroxidase(POD)activity in the leaves were significantly increased.The relative expression levels of antioxidant enzyme genes AtSOD and AtPOD were also sig-nificantly up-regulated.The above results indicated that soybean GmMYC2L gene could enhance salt tolerance of plants through antioxidant pathway.The results of this study provide candidate genes for genetic improvement of salt toler-ance in soybean and other crops.
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